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Cloning and sequencing of xylanase gene from
Bacillus subtilis
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Khadijeh Asadi-Farsani, Abbas Doosti* and Abbas Mokhtari-Farsani |
Biotechnology Research
Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
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Abstract |
Xylans are the principle
non-starch polysaccharides of wheat in poultry diets which can increase
the
intestinal
viscosity and decrease the nutrient absorption. Xylanases
catalyze the hydrolysis of xylans. The aim of present study was to clone
xylanase gene in pGEM vector and sequencing of this gene from the
Bacillus subtilis. Genomic DNA from B. subtilis was
isolated and amplified by PCR using Xylanase specific PCR
primers. Then xylanase was cloned by T/A cloning technique and
transformed into TOP10 E.
coli cells. Finally, after sequencing, xylanase
sequence
similarity was checked using nucleotide BLAST analysis. The results of present study showed that
xylanase was successfully cloned in pGEM vector. Sequencing
confirmed that xylanase was cloned and the length of xylanase
was 661 bp. BLAST search showed that the sequence of xylanase
gene of the B. subtilis has 99% homology with other records
existing in GenBank.
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Keywords:
Xylanase; Bacillus subtilis; cloning and sequencing;
probiotic;
poultry diets |
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To cite this article:
Asadi-Farsani K, A Doosti and A Mokhtari-Farsani, 2015.
Cloning
and sequencing of xylanase
gene from Bacillus subtilis.
Res.
Opin. Anim. Vet. Sci., 5(5): 215-218. |
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