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Identification of different allelic forms in exon 1 of FSHβ in Iranian
Baluchi and Naeini sheep breeds by PCR-SSCP
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Hosein Lameei, Kian Pahlevan Afshari* and
Mehran Abozar |
Department
of Animal Science, Abhar Branch, Islamic Azad University, Abhar, Iran
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Abstract |
Follicle-stimulating hormone (FSH) is fundamental for gamete maturation
and
is known to regulate the production of several growth factors that play
a critical role in primordial follicle activation and growth.
The aim of this study was to evaluate the exon 1
polymorphism of FSHβ gene in Naeinian and Baluchi sheep breeds. Blood
samples were randomly collected from 109 both of two sheep breeds (70
Naeinian sheep and 39 Baluchi sheep). DNA was extracted using modified
salting out method. A fragment of 220 bp from exon 1 region was
amplified using a pair of specific primers by polymerase chain reaction
(PCR). In order to detection of allelic different forms were used from
PCR-SSCP and direct sequencing techniques. PCR-SSCP analysis
demonstrated eight different banding patterns from exon 1 region of FSHβ
gene. After genetic analysis on sequencing results and comparing with
the sequence of ovine FSHβ published on GeneBank (accession number
S64745.1), a nucleotide insertion in non-coding region of FSH (110
A-T→ACT) was detected. The obtained results from both of two techniques
revealed that this region has different allelic forms.
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Keywords:
PCR-SSCP;
FSHβ; polymorphisms; Baluchi; Naeinian |
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To cite this article:
Lameei H,
KP Afshari and M Abozar, 2015.
Identification of different
allelic forms in exon 1 of FSHβ in Iranian Baluchi and Naeini sheep
breeds by PCR-SSCP.
Res.
Opin. Anim. Vet. Sci., 5(9): 360-365. |
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