E-ISSN 2223-0343

Identification of different allelic forms in exon 1 of FSHβ in Iranian Baluchi and Naeini sheep breeds by PCR-SSCP

Hosein Lameei, Kian Pahlevan Afshari* and Mehran Abozar

Department of Animal Science, Abhar Branch, Islamic Azad University, Abhar, Iran

 
Abstract

Follicle-stimulating hormone (FSH) is fundamental for gamete maturation and is known to regulate the production of several growth factors that play a critical role in primordial follicle activation and growth. The aim of this study was to evaluate the exon 1 polymorphism of FSHβ gene in Naeinian and Baluchi sheep breeds. Blood samples were randomly collected from 109 both of two sheep breeds (70 Naeinian sheep and 39 Baluchi sheep). DNA was extracted using modified salting out method. A fragment of 220 bp from exon 1 region was amplified using a pair of specific primers by polymerase chain reaction (PCR). In order to detection of allelic different forms were used from PCR-SSCP and direct sequencing techniques. PCR-SSCP analysis demonstrated eight different banding patterns from exon 1 region of FSHβ gene. After genetic analysis on sequencing results and comparing with the sequence of ovine FSHβ published on GeneBank (accession number S64745.1), a nucleotide insertion in non-coding region of FSH (110 A-T→ACT) was detected. The obtained results from both of two techniques revealed that this region has different allelic forms.

Keywords: PCR-SSCP; FSHβ; polymorphisms; Baluchi; Naeinian
 
To cite this article: Lameei H, KP Afshari and M Abozar, 2015. Identification of different allelic forms in exon 1 of FSHβ in Iranian Baluchi and Naeini sheep breeds by PCR-SSCP. Res. Opin. Anim. Vet. Sci., 5(9): 360-365.
 
 
 

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