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Identification of Campylobacter spp. with multiplex PCR assay in healthy and diarrheic dogs

Hamed Zamankhan Malayeri1, Shahram Jamshidi1*, Taghi Zahraei Salehi2, Bahar Nayeri Fasaei2 and Iraj Ashrafi Tamaei2

1Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
2Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

 
Abstract

Campylobacter is an important and common cause of human gastroenteritis. Dogs are considered as reservoir of Campylobacter spp. and can infect people and other animals by shedding these organisms in stool. In this study, prevalence of Campylobacter and its species in diarrheic and healthy dogs were determined. Faecal samples from 75 healthy and 75 diarrheic dogs were acquired and assessed for the presence of campylobacter species by Multiplex PCR. Campylobacter spp. prevalence was significantly more in diarrheic group (45.3% and 64.0% in healthy and diarrheic groups respectively). Out of 82 positive samples, 52.4% contained only C. jejuni, and 37.8% C. upsaliensis, whereas 9.8% harboured both species. The age had a significant effect on Campylobacter infection and the highest shedding prevalence was observed in young diarrheic dogs (less than 6 months). According to the results of this study, diarrheic and young pets should be considered as important source of zoonotic pathogen for humans.

Keywords: Campylobacter spp.; diarrhoea; dogs; multiplex PCR
 
To cite this article: Malayeri HZ, S Jamshidi, TZ Salehi, BN Fasaei and IA Tamaei, 2014. Identification of Campylobacter spp. with multiplex PCR assay in healthy and diarrheic dogs. Res. Opin. Anim. Vet. Sci., 4(9): 489-495.

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