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PRINT: ISSN 2221-1896
ONLINE : ISSN 2223-0343
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Identification of Campylobacter spp. with multiplex PCR assay in
healthy and diarrheic dogs
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Hamed Zamankhan Malayeri1, Shahram Jamshidi1*,
Taghi
Zahraei Salehi2, Bahar Nayeri Fasaei2
and Iraj Ashrafi Tamaei2 |
1Department of Internal Medicine, Faculty of
Veterinary Medicine, University
of Tehran,
Tehran,
Iran
2Department of Microbiology and Immunology, Faculty
of Veterinary Medicine, University
of Tehran,
Tehran,
Iran
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Abstract |
Campylobacter is an important and common cause of human
gastroenteritis. Dogs are considered as reservoir of Campylobacter
spp. and can infect people and other animals by shedding these organisms
in stool. In this study, prevalence of Campylobacter and its species in diarrheic and healthy
dogs
were determined.
Faecal samples from 75
healthy and 75 diarrheic dogs were acquired and assessed for the
presence of campylobacter species by
Multiplex PCR.
Campylobacter
spp. prevalence was significantly more in diarrheic group (45.3% and
64.0% in healthy and diarrheic groups respectively). Out of 82 positive
samples, 52.4% contained only C. jejuni, and 37.8% C.
upsaliensis, whereas 9.8% harboured both species.
The age had a significant effect on Campylobacter
infection and the highest shedding prevalence was observed in young
diarrheic dogs (less than 6 months).
According to the results of this study, diarrheic and young pets should
be considered as important source of zoonotic pathogen for humans.
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Keywords:
Campylobacter spp.; diarrhoea; dogs; multiplex PCR |
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To cite this article:
Malayeri HZ, S Jamshidi, TZ
Salehi, BN Fasaei and IA Tamaei, 2014.
Identification of
Campylobacter spp. with multiplex PCR assay in healthy and diarrheic
dogs.
Res.
Opin. Anim. Vet. Sci., 4(9): 489-495. |
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