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Effect of adding orange juice into semen diluents on quality and storage ability of cocks' semen
Hazim J. Al-Daraji
University of Baghdad, College of Agriculture, Department of Animal Production, Baghdad, Iraq

The effect of adding orange juice into the extender of cocks' semen on mass motility, individual motility, dead and abnormal spermatozoa and acrosomal abnormalities were studied. A total of 60 White layer cocks, 32 weeks of age, were randomly divided into 6 groups of 10 cocks in this experiment. Semen samples for each treatment group were collected on a weekly basis during the whole experimental period which lasted 8 weeks. The treatment groups were; T1- the control (fresh semen); T2- the semen extended 1 : 1 with Al-Daraji 2 extender (AD2E) alone, whereas T3, T4, T5 and T6 represented semen samples extended with AD2E extender and supplemented with 1, 4, 7 or 10 ml of orange juice/100 ml extender. Results revealed that after 0, 24, 48 or 72 h in vitro storage, the supplementation of cocks semen extender with 7 and 10 ml orange juice/100 ml of extender (T5, T6) caused significant (P<0.05) increases in mass motility and individual motility of spermatozoa and significant decreases (P<0.05) in percentages of dead spermatozoa, abnormal spermatozoa and acrosomal abnormalities compared with control group. However, T2, T3 and T4 groups showed significant improvements in all of theses parameters in comparison with control group. Furthermore, there were no significant differences between T5 and T6 and between T2, T3 and T4 as regards all characteristics included in this study. In conclusion, the supplementation of orange juice into semen extender plays an important role in protecting spermatozoa against the harmful effects of lipid peroxidation during in vitro storage of cocks' semen for up to 72 h.

Keywords: Orange juice; semen quality; liquid storage; cocks
To cite this article: Al-Daraji HJ, 2012. Effect of adding orange juice into semen diluents on quality and storage ability of cocks' semen. Res. Opin. Anim. Vet. Sci., 2(9), 485-489.

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