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PRINT: ISSN 2221-1896
ONLINE : ISSN 2223-0343
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Isolation and identification of
avian
leukosis virus in Shouguang chickens
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Jing Kai Xin, Qing Yin, Yu Sheng Li, Yuan Yuan Jing
and Jia Qian Chai* |
Key Laboratory of Animal
Biotechnology and Disease Control and Prevention of Shandong Province,
College of Veterinary Medicine, Shandong Agricultural University, Taian
271018, People’s Republic of China
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Abstract |
Serum and cloacal samples
from suspected Shouguang chickens were collected for detection of avian
leukosis. The percentage of anti-ALV-A/B was 6.73, 2.27 and 1.28% in
parental lines, hybrid lines and one day old birds of Shouguang chicken
respectively. Moreover, high percentages of p27 antigen-positive
chickens were found in all 3 lines.
Proviral DNA samples of
the ALV-B extracted from the chickens and the virus-infected DF-1 cells
were used as templates for PCR. Through the sequenced of ALV-B-SG12-gp85
and compared the results with 12 strains of ALV-A and 6 strains of
ALV-B. It was confirmed that the ALV isolated from Shouguang chickens
was ALV-B and might be a common origin to JS-B1203 (GenBank: KC282891.1)
in China. One or
more small tumour nodules were observed on the surface of livers in the
early stage in Shouguang chickens while the scattered tumour nodules
were found on enlarged livers, spleens and kidneys in the late stage.
Besides, tumour nodules were also detected in other
viscera. Histologically,
visceral tissues
mainly contained myeloid cells with acidophilic granules with
densely-arranged lymphoblast while normal cells were squeezed and even
destroyed by these tumour cells. The increased number of tumour cells
and intra and extra-cellular hydropic degeneration might be responsible
for the enlargement of viscera organs.
We concluded that the ALV isolated from Shouguang chickens was ALV-B and
have a high common origin to JS-B1203 (GenBank: KC282891.1) in China.
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Keywords:
Avian leucosis; serology;
isolation; identification; pathology |
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To cite this article:
Xin JK, Q Yin, YS Li, YY
Jing and JQ Chai,
2014.
Isolation and identification of avian leukosis virus in Shouguang
chickens.
Res.
Opin. Anim. Vet. Sci., 4(8): 462-466. |
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