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Isolation and identification of avian leukosis virus in Shouguang chickens

Jing Kai Xin, Qing Yin, Yu Sheng Li, Yuan Yuan Jing and Jia Qian Chai*

Key Laboratory of Animal Biotechnology and Disease Control and Prevention of Shandong Province, College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, People’s Republic of China

 
Abstract

Serum and cloacal samples from suspected Shouguang chickens were collected for detection of avian leukosis. The percentage of anti-ALV-A/B was 6.73, 2.27 and 1.28% in parental lines, hybrid lines and one day old birds of Shouguang chicken respectively. Moreover, high percentages of p27 antigen-positive chickens were found in all 3 lines. Proviral DNA samples of the ALV-B extracted from the chickens and the virus-infected DF-1 cells were used as templates for PCR. Through the sequenced of ALV-B-SG12-gp85 and compared the results with 12 strains of ALV-A and 6 strains of ALV-B. It was confirmed that the ALV isolated from Shouguang chickens was ALV-B and might be a common origin to JS-B1203 (GenBank: KC282891.1) in China. One or more small tumour nodules were observed on the surface of livers in the early stage in Shouguang chickens while the scattered tumour nodules were found on enlarged livers, spleens and kidneys in the late stage. Besides, tumour nodules were also detected in other viscera. Histologically, visceral tissues mainly contained myeloid cells with acidophilic granules with densely-arranged lymphoblast while normal cells were squeezed and even destroyed by these tumour cells. The increased number of tumour cells and intra and extra-cellular hydropic degeneration might be responsible for the enlargement of viscera organs. We concluded that the ALV isolated from Shouguang chickens was ALV-B and have a high common origin to JS-B1203 (GenBank: KC282891.1) in China.

Keywords: Avian leucosis; serology; isolation; identification; pathology
 
To cite this article: Xin JK, Q Yin, YS Li, YY Jing and JQ Chai, 2014. Isolation and identification of avian leukosis virus in Shouguang chickens. Res. Opin. Anim. Vet. Sci., 4(8): 462-466.
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

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