|
PRINT: ISSN 2221-1896
ONLINE : ISSN 2223-0343
|
|
|
Evaluation of the wound healing activity of methanolic extract of
Tragopogon porrifolius in rat
|
Mohammad Hashemnia1*, Moosa Javdani2, Zahra
Nikousefat2,
Fatemeh Hoseinpour1
and Shabnam Kakaei1 |
1Department
of Pathology, School of Veterinary Medicine, Razi University, Kermanshah,
Iran; 2Department of Clinical Sciences, School of Veterinary
Medicine, Razi University, 6715685414, Kermanshah, Iran
|
|
Abstract |
This study was undertaken to evaluate the wound healing properties of
Tragopogon porrifolius (TP) on cutaneous wounds in rat. A
full-thickness cutaneous defect (2×2cm) was induced on the back of 60
Sprague-Dawley rats. The animals were randomly divided into four equal
groups, treated with 1ml basal cream (placebo group), 1ml tetracycline
(3%), 1ml TP 10% and untreated (control). Five animals of each group
were euthanized at each of 7, 14 and 21 days post-injury (DPI) and
wounds were evaluated through histopathological analyses. Treated
animals with TP showed a significant reduction in the wound surface area
at 14 and 21 DPI compared to other groups. In addition, treatment with
TP reduced the number of lymphocytes and enhanced the number of
fibroblasts at the earlier stages of wound healing and increased number
of fibrocytes at the later stages of wound healing. TP significantly
improved alignment of the healing tissue, re-epithelilization and
epithelial formation, enhanced maturity of the collagen fibres and
fibroblasts and large capillary-sized blood vessels.
The present study demonstrated that the methanolic extract of
T. porrifolius
promoted wound healing activity in animal as a preclinical study.
These results showed that application of TP extract on wounds induces
significant wound contraction and accelerated healing
and it may be suggested for treating various types of wounds in animal
and human beings.
|
Keywords:
Tragopogon porrifolius;
methanolic extract; wound healing; rat |
|
To cite this article:
Hashemnia M, M Javdani, Z Nikousefat,
F Hoseinpour
and S Kakaei,
2014.
Evaluation of the wound healing activity of methanolic extract of
Tragopogon porrifolius in rat.
Res.
Opin. Anim. Vet. Sci., 4(8): 446-452. |
|
|