PRINT: ISSN 2221-1896
ONLINE : ISSN 2223-0343

Evaluation of the wound healing activity of methanolic extract of Tragopogon porrifolius in rat

Mohammad Hashemnia1*, Moosa Javdani2, Zahra Nikousefat2, Fatemeh Hoseinpour1 and Shabnam Kakaei1

1Department of Pathology, School of Veterinary Medicine, Razi University, Kermanshah, Iran; 2Department of Clinical Sciences, School of Veterinary Medicine, Razi University, 6715685414, Kermanshah, Iran


This study was undertaken to evaluate the wound healing properties of Tragopogon porrifolius (TP) on cutaneous wounds in rat. A full-thickness cutaneous defect (2×2cm) was induced on the back of 60 Sprague-Dawley rats. The animals were randomly divided into four equal groups, treated with 1ml basal cream (placebo group), 1ml tetracycline (3%), 1ml TP 10% and untreated (control). Five animals of each group were euthanized at each of 7, 14 and 21 days post-injury (DPI) and wounds were evaluated through histopathological analyses. Treated animals with TP showed a significant reduction in the wound surface area at 14 and 21 DPI compared to other groups. In addition, treatment with TP reduced the number of lymphocytes and enhanced the number of fibroblasts at the earlier stages of wound healing and increased number of fibrocytes at the later stages of wound healing. TP significantly improved alignment of the healing tissue, re-epithelilization and epithelial formation, enhanced maturity of the collagen fibres and fibroblasts and large capillary-sized blood vessels. The present study demonstrated that the methanolic extract of T. porrifolius promoted wound healing activity in animal as a preclinical study. These results showed that application of TP extract on wounds induces significant wound contraction and accelerated healing and it may be suggested for treating various types of wounds in animal and human beings.

Keywords: Tragopogon porrifolius; methanolic extract; wound healing; rat
To cite this article: Hashemnia M, M Javdani, Z Nikousefat, F Hoseinpour and S Kakaei, 2014. Evaluation of the wound healing activity of methanolic extract of Tragopogon porrifolius in rat. Res. Opin. Anim. Vet. Sci., 4(8): 446-452.

Home  |  Archive  |  Instructions  |  Submission  |  Editorial Board  |  Sample Paper

All rights reserved ©