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Tumor necrosis factor receptor type-1 is not expressed on granulosa cells of pre-ovulatory follicles in sheep

Mohammad Yahyaei1,4, Mohammad Khorvash1, Hamid R. Rahmani1, Mahdi Khodaei Motlagh2 and Hamid R. Mohammadnia3

Department of Animal Science, College of Agriculture, Isfahan University of Technology, Isfahan, Iran1, Department of Animal Science, Faculty of Agriculture, Arak university, Arak 38156-8-8349. Iran2, Faculty of Veterinary medicine, Ferdowsi University of Mashhad (FUM), Iran3, PhD student of Nanobiotechnology, Life Science Engineering Group, Department of New Sciences and Technologies, University of Tehran4

 
Abstract

This study was conducted to examine the expression of tumor necrosis factor receptor type1 (TNFR-I) on granulosa cells of pre-ovulatory follicles in sheep. In all ewes (n=15), estrus was synchronized with the Controlled Internal Drug Release CIDR (12 day). The granulosa cells were collected from pre-ovulatory follicle 48 hours after CIDR withdrawal. Expression of mRNA was investigated using a reverse transcriptase-polymerase chain reaction (RT-PCR). Total RNA was extracted from cells and then reverse-transcribed to synthesize cDNA and PCR-amplified using specific oligonucleotide primers. Result of present study indicates TNFR-1 was not expressed at the mRNA level. In general, this finding provides further insight into the proposed physiologic roles of TNFα in ovarian function.

Keywords: Sheep; TNFR-I; Pre-ovulatory Follicles; granulosa cells; gene expression
 
To cite this article: Yahyaei M, M Khorvash, HR Rahmani, MK Motlagh and HR Mohammadnia, 2013. Tumor necrosis factor receptor type-1 is not expressed on granulosa cells of pre-ovulatory follicles in sheep. Res. Opin. Anim. Vet. Sci., 3(8), 257-260.
 
 
 
 
 

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