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PRINT: ISSN 2221-1896
ONLINE : ISSN 2223-0343
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Tumor
necrosis factor receptor type-1 is not expressed on granulosa cells of
pre-ovulatory follicles in sheep |
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Mohammad Yahyaei1,4, Mohammad Khorvash1, Hamid R.
Rahmani1, Mahdi Khodaei Motlagh2 and Hamid R.
Mohammadnia3
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Department
of Animal Science, College of Agriculture, Isfahan University of
Technology, Isfahan, Iran1, Department of Animal Science,
Faculty of Agriculture, Arak university, Arak 38156-8-8349. Iran2,
Faculty of Veterinary medicine, Ferdowsi University of Mashhad (FUM),
Iran3, PhD student of Nanobiotechnology, Life Science
Engineering Group, Department of New Sciences and Technologies,
University of Tehran4
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Abstract |
This study was conducted to
examine the expression of tumor necrosis factor receptor type1 (TNFR-I)
on granulosa cells of pre-ovulatory follicles in sheep. In all ewes
(n=15), estrus was synchronized with the Controlled Internal Drug
Release CIDR (12 day). The granulosa cells were collected from
pre-ovulatory follicle 48 hours after CIDR withdrawal. Expression of
mRNA was investigated using a reverse transcriptase-polymerase chain
reaction (RT-PCR). Total RNA was extracted from cells and then
reverse-transcribed to synthesize cDNA and PCR-amplified using specific
oligonucleotide primers. Result of present study indicates TNFR-1 was
not expressed at the mRNA level. In general, this finding provides
further insight into the proposed physiologic roles of TNFα in ovarian
function.
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Keywords:
Sheep; TNFR-I; Pre-ovulatory
Follicles; granulosa cells; gene expression |
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To cite this article:
Yahyaei M,
M Khorvash, HR Rahmani, MK
Motlagh and HR Mohammadnia, 2013.
Tumor necrosis factor
receptor type-1 is not expressed on granulosa cells of pre-ovulatory
follicles in sheep.
Res.
Opin. Anim. Vet. Sci., 3(8), 257-260. |
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