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Morphometric analysis of low level laser therapy (780 nm) in transected sciatic nerve regeneration after end to end neurorrhaphy in rabbit
Ashrafzadeh Takhtfooladi M1*, Sharifi D2, Mokmeli S3, Abedi G1, Mortazavi P4, Sohrabi Haghdoust I4, Fattahian H1 and Asghari A1
1Department of Surgery, Faculty of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, Iran; 2Department of Surgery & Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran; 3Department of Medical Laser, Milad Hospital, Tehran, Iran; 4Department of Pathology, Faculty of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
 
Abstract

Peripheral nerve transections represent common clinical occurrence. There are two main requirements for a good clinical outcome after these lesions. The first is surgical treatment so as to make possible the regeneration of the transected nerve fibers toward their original periphery. The second important requirement for successful recovery after a nerve lesion is postoperative rehabilitation treatment. In fact, although the surgical techniques have proved to lead almost always to the regeneration of nerve fibers, the degree of recovery can be highly variable among patients. The objective of this study was to investigate whether 780 nm low level lasers could stimulate the healing process in the sciatic nerve regeneration in rabbits submitted to complete transection through morphometric analysis. Twenty adult white New Zealand male rabbits were used, where the injury of the type neurotmesis of the right sciatic nerve under general anesthesia (intramuscular ketamine/xylazine and atropine sulfate) was approximated using prolene 6-0. The rabbits were randomly distributed in 2 groups with 10 rabbits each. In group I, Arsenate of Gallium Laser was used with the extension of wave of 780 nm, 10 mW powers with 1 J/cm2 irradiation with 10 seconds for each Cm2, in the pulsed form. The laser therapy was initiated on the post-surgical first day with application 10 minutes for each rabbit in group I, once a day for 15 days. Rabbits in group II had not given treatment (control group). The samples of transected nerves were collected and prepared for morphometric analysis on the 90 days, they were analyzed the average number of total counted fibers and the mean fiber density on proximal, intermediate and distal segments. On group I and II, the average number of total counted fibers and the mean fiber density on proximal, intermediate and distal segments was significant in each group. The differences found for total number of counted fibers and for fibers density between proximal segments of groups I and II were not significant (p=0.31 and p=0.17, respectively). On intermediate segments (injury site), the total number of counted fibers was significantly higher in group I (p=0.05). On distal segments, both the total number and the density of fibers were significantly higher in group I (p=0.03 and 0.001, respectively). The irradiation with low level laser (780 nm) influenced positively the regeneration of the sciatic nerve in rabbits after being injured and end to end anastomosis, becoming the nerve recovery more rapid and efficient.

Keywords: Low level laser therapy; morphometric analysis; nerve regeneration; sciatic nerve; rabbit
 
To cite this article: Takhtfooladi MA, Sharifi D, Mokmeli S, Abedi G, Mortazavi P, S Haghdoust I, Fattahian H and Asghari A, 2012. Morphometric analysis of low level laser therapy (780 nm) in transected sciatic nerve regeneration after end to end neurorrhaphy in rabbit. Res. Opin. Anim. Vet. Sci., 2(8), 480-484.
 
 
 
 
 
 
 
 
 
 
 
 
 

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