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PRINT: ISSN 2221-1896
ONLINE : ISSN 2223-0343
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Morphometric analysis of low level laser therapy (780 nm) in transected
sciatic nerve regeneration after end to end neurorrhaphy in rabbit |
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Ashrafzadeh Takhtfooladi M1*, Sharifi D2, Mokmeli
S3, Abedi G1, Mortazavi P4, Sohrabi
Haghdoust I4, Fattahian H1 and Asghari A1 |
1Department
of Surgery, Faculty of Specialized Veterinary Science, Science and
Research Branch, Islamic Azad University, Tehran, Iran; 2Department
of Surgery & Radiology, Faculty of
Veterinary
Medicine, University of Tehran, Tehran, Iran; 3Department
of Medical Laser, Milad Hospital, Tehran, Iran; 4Department
of Pathology, Faculty of Specialized Veterinary Science, Science and
Research Branch, Islamic Azad University, Tehran, Iran |
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Abstract |
Peripheral nerve transections represent common clinical occurrence.
There are two main requirements for a good clinical outcome after these
lesions. The first is surgical treatment so as to make possible the
regeneration of the transected nerve fibers toward their original
periphery. The second important
requirement for successful recovery after a nerve lesion is
postoperative rehabilitation treatment. In fact, although the surgical
techniques have proved to lead almost always to the regeneration of
nerve fibers, the degree of recovery can be highly variable among
patients.
The objective of this study was to investigate whether 780 nm low level
lasers could stimulate the healing process in the sciatic nerve
regeneration in rabbits submitted to complete transection through
morphometric analysis.
Twenty adult white New Zealand
male rabbits were used, where the injury of the type neurotmesis of the
right sciatic nerve under general anesthesia (intramuscular
ketamine/xylazine and atropine sulfate) was approximated using prolene
6-0. The rabbits were randomly distributed in 2 groups with 10 rabbits
each. In group I, Arsenate of Gallium Laser was used with the extension
of wave of 780 nm, 10 mW powers with 1 J/cm2 irradiation with
10 seconds for each Cm2, in the pulsed form. The laser
therapy was initiated on the post-surgical first day with application 10
minutes for each rabbit in group I, once a day for 15 days. Rabbits in
group II had not given treatment (control group). The samples of
transected nerves were collected and prepared for morphometric analysis
on the 90 days, they were analyzed the average number of total counted
fibers and the mean fiber density on proximal, intermediate and distal
segments. On group I and II, the average number of total counted fibers
and the mean fiber density on proximal, intermediate and distal segments
was significant in each group. The differences found for total number of
counted fibers and for fibers density between proximal segments of
groups I and II were not significant (p=0.31 and p=0.17, respectively).
On intermediate segments (injury site), the total number of counted
fibers was significantly higher in group I (p=0.05). On distal segments,
both the total number and the density of fibers were significantly
higher in group I (p=0.03 and 0.001, respectively). The irradiation with
low level laser (780 nm) influenced positively the regeneration of the
sciatic nerve in rabbits after being injured and end to end anastomosis,
becoming the nerve recovery more rapid and efficient.
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Keywords:
Low level laser therapy; morphometric analysis; nerve regeneration;
sciatic nerve; rabbit |
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To cite this article:
Takhtfooladi MA, Sharifi D, Mokmeli S, Abedi G, Mortazavi P, S Haghdoust
I, Fattahian H and Asghari A,
2012.
Morphometric analysis of low level laser therapy (780 nm) in transected
sciatic nerve regeneration after end to end neurorrhaphy in rabbit.
Res.
Opin. Anim. Vet. Sci., 2(8), 480-484. |
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