This work was an attempt to reveal the effects of a Jordanian cigarette smoke and pure L-nicotine on the contractile activity of tracheal and aortic smooth muscles, and isolated perfused heart of albino rat. In tissue bath experiments, cigarette smoke extracts (CSE) and L-nicotine induced biphasic change in the tone of isolated precontracted-tracheal and aortic smooth muscles. In the case of isolated perfused heart, CSE and L-nicotine induced in most concentrations a significant concentration dependent reduction of the force and the rate of contraction of the perfused heart. One gram of cigarette tobacco produces an equivalent of 4.8 mg L-nicotine in cigarette smoke extract.