E-ISSN 2223-0343

Molecular characterization and Bioinformatics analysis of 31kDa major outer membrane protein gene of Brucella melitensis, Malaysian isolate

Omer Khazaal Sallou1&4*, Ramlan Bin Mohamed2, Mohd Mokhtar B. Arshad1, Hirzahida Mohd. Padil3, Shuhaila Mat Sharani3, Hardy Abu Daud1, Kalthum Bt. Hashim1, Mohd Azam Khan and B. Goriman Khan1

1Faculty of Veterinary Medicine, University Malaysia Kelantan, Karung Berkunci 36, Pengkalan Chepa, 16100, Koto Bharu, Kelantan, Malaysia, 2Veterinary Research Institute, 59 Jalan Sultan Azlan Shah 31400 Ipoh, Perak Darul Ridzuan, Malaysia, 3Malaysia Genome Institute, Ministry of Science Technology and Innovation, Jalan Bangi, 43000 Kajang, Selangor Darul Ehsan, Malaysia; 4Department of Internal and Preventive Medicine, College of Veterinary Medicine, University of Mosul, Iraq


Brucellosis research is currently focused on molecular characterization of omp31 gene from different Brucella species with the ultimate goal of development suitable, safe, and effective vaccine or specific diagnostic test. In this study, the molecular characterization, amino acid variations, protein structure and function of omp31 from B. melitensis, Malaysian isolate was investigated by using Bioinformatics approach. The bacterium was reactivated on selective medium base. Then, the bacterial growth was examined by Gram stain and biochemical tests. The omp31 gene, encoding a major outer membrane protein, was PCR-amplified by using primers selected according to the B. melitensis strain 152 published sequences. The nucleotide and predicted amino acid sequences were analysed, using Bioinformatics software and tools, which include domain analysis, homology search, multiple sequence alignment and constructing phylogenetic tree. The results show the cDNA sequence of omp31 consists of 690 base pairs, which translated into its open reading frame (ORF) sequence of 229 amino acids in size. From the domain analyses, the omp31 has beta-barrel structure that acts as a transporter for ions and small molecules, which cannot diffuse across a cellular membrane. Moreover, it has Beta-ketoacyl synthase domain that produces fatty acids of various lengths for use by the organism. The phylogenetic analysis of omp31 and its homologous sequences revealed that omp31 is conserved among all the B. melitensis strains and it has 100% similarity with omp31 of B. melitensis M28 (Chinese strain). In conclusion, the omp31 has high sequence homology with published sequences, suggesting that omp31 of B. melitensis is highly conserved. The domain analysis provides further functionality of omp31, in addition to its antigenic characteristic. Accordingly, the omp31 might be an interesting candidate for development of omp31 based specific diagnostic test against goat Brucellosis caused by B. melitensis.

Keywords: Brucella melitensis; omp31; Bioinformstics tools; Brucellosis
To cite this article: Sallou OK, RB Mohamed, MMB Arshad, HM Padil, SM Sharani, HA Daud, KB Hashim, MA Khan and BG Khan, 2016. Molecular characterization and Bioinformatics analysis of 31kDa major outer membrane protein gene of Brucella melitensis, Malaysian isolate. Res. Opin. Anim. Vet. Sci., 6(7): 218-223.

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