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Molecular characterization and Bioinformatics analysis
of 31kDa major outer membrane protein
gene
of Brucella melitensis, Malaysian
isolate
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Omer Khazaal Sallou1&4*, Ramlan Bin
Mohamed2, Mohd Mokhtar B. Arshad1,
Hirzahida Mohd. Padil3, Shuhaila Mat
Sharani3, Hardy Abu Daud1, Kalthum Bt. Hashim1,
Mohd Azam Khan and B. Goriman Khan1 |
1Faculty of Veterinary Medicine, University Malaysia
Kelantan, Karung Berkunci 36, Pengkalan Chepa, 16100, Koto Bharu,
Kelantan, Malaysia, 2Veterinary Research Institute, 59 Jalan
Sultan Azlan Shah 31400 Ipoh, Perak Darul Ridzuan, Malaysia, 3Malaysia
Genome Institute, Ministry of Science Technology and Innovation, Jalan
Bangi, 43000 Kajang, Selangor Darul Ehsan, Malaysia;
4Department of Internal and Preventive Medicine,
College of Veterinary Medicine, University of Mosul, Iraq
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Abstract |
Brucellosis research is
currently focused on molecular characterization of omp31 gene
from different Brucella species with the ultimate goal of development
suitable, safe, and effective vaccine or specific diagnostic test.
In this study,
the
molecular characterization,
amino acid variations, protein structure and function
of
omp31 from B. melitensis,
Malaysian isolate was investigated by using Bioinformatics
approach.
The bacterium was
reactivated on selective medium base. Then, the bacterial growth was
examined by Gram stain and biochemical tests. The omp31 gene,
encoding a major outer membrane protein, was PCR-amplified by using
primers selected according to the B. melitensis
strain 152
published sequences. The nucleotide and
predicted amino acid sequences were analysed, using Bioinformatics
software and tools, which include
domain analysis, homology search, multiple sequence alignment and
constructing phylogenetic tree. The results show the cDNA
sequence of omp31 consists of 690
base pairs, which translated into its open reading frame (ORF)
sequence of 229 amino acids in size. From the domain analyses, the
omp31 has beta-barrel structure that acts as a transporter
for ions and small molecules, which cannot diffuse across
a cellular membrane. Moreover, it has Beta-ketoacyl synthase domain that
produces fatty
acids of various lengths for use by the organism. The phylogenetic
analysis of omp31 and its homologous sequences revealed that
omp31 is conserved among
all the B. melitensis strains and it has 100% similarity with
omp31 of
B. melitensis M28 (Chinese strain).
In conclusion, the omp31
has high sequence homology with published sequences,
suggesting that
omp31
of
B. melitensis
is highly conserved.
The domain analysis provides further functionality of omp31, in
addition to its antigenic characteristic. Accordingly, the omp31
might be an interesting candidate for
development of omp31 based specific diagnostic test
against goat Brucellosis caused by B. melitensis.
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Keywords:
Brucella melitensis; omp31; Bioinformstics tools; Brucellosis |
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To cite this article:
Sallou OK, RB Mohamed,
MMB Arshad,
HM
Padil, SM Sharani, HA Daud, KB Hashim, MA Khan and BG Khan, 2016.
Molecular characterization and Bioinformatics
analysis of 31kDa major outer
membrane protein gene
of Brucella melitensis, Malaysian
isolate.
Res.
Opin.
Anim. Vet. Sci., 6(7): 218-223. |
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