|
|
|
|
Antimicrobial susceptibility of large intestinal
Escherichia coli isolates from
cattle and sheep abattoir samples
|
Buyukunal EB1*, Mahmood KH1 and Bal MA2 |
1Department of Biology;
2Department of Animal Science, Kahramanmaras Sutcu Imam
University, 46100 Kahramanmaras, Turkey
|
|
Abstract |
Escherichia coli reside in gastrointestinal tracts of warm-blooded
animals and can serve as an indicator organism for monitoring antibiotic
resistance that is considered an important global issue. In this study,
E. coli (n=49) were isolated
from 40 samples of colon contents consisting from 20 cattle and 20 sheep
carcasses and identified based on standard biochemical tests. The
identification was also verified by chromogenic media. All isolates were
tested against 13 antibiotic agents by disc diffusion method for
determining antimicrobial susceptibilities. Double Disk Synergy test was
applied for the detection of the ESBL producing isolates. Nearly all
isolates were found to be susceptible to most of the antibiotics. The
resistance to tested antibiotics was low; only 6 (12.24%) of 49
E. coli isolates were
resistant to at least one antibiotic agent. Furthermore, different
resistance rates to antibiotics were observed for cattle and sheep
isolates. Among 28 isolates from cattle, the highest resistance levels
were observed for trimethoprim-sulfamethoxazole (n=3; 6.12%), imipenem
(n=2; 4.08%) and cefpodoxime (n=1; 3.57), while only
ciprofloxacin
resistance (n=2 9.52%) was observed among 21 isolates from sheep. This
is the first report about imipenem resistant
E. coli of animal origin from
Turkey. Furthermore, no ESBL positive isolate was detected at all. In
conclusion, continuous and strategic surveillance of antimicrobial
resistant bacteria in livestock is essential to suppress further
dissemination of these bacteria into Turkish society.
|
Keywords:
Abattoir; antibiotics; Enterobacteriaceae;
E. coli |
|
To cite this article:
Buyukunal EB, Mahmood KH and
Bal MA, 2016.
Antimicrobial susceptibility of
large intestinal
Escherichia coli isolates from cattle and sheep abattoir samples.
Res.
Opin. Anim. Vet. Sci., 6(7): 211-217. |
|
|