Nicotine has been suggested to have deleterious effects on the outcome of pregancy. This study investigated the effects of maternal nicotine and vitamin C during pregnancy on the expression of laminin-α5 in kidneys of offspring. In this study, eighteen female BALB/c mice, after pregnancy confirmation, were randomly divided into three groups: experimental group 1 (T1) received 0.3 mg/kg/day nicotine and experimental group 2 (T2) received simultaneously 0.3 mg/kg/day nicotine and 9 mg/kg/day vitamin C and control group (T3) received 3 ml/kg/day normal saline parallel to other groups since the 6th day of gestation to the end of prenatal life. At birth, laminin-α5 content of the renal cortex, glomerulus and convoluted tubules were determined immunohistochemically (IHC) and real-time polymerase chain reaction (RT-PCR). Maternal nicotine reduced birth weight but increased kidney weight although not significantly (P>0.05). The IHC results showed that laminin-α5 decreased significantly (P<0.05) in the renal cortex, glomerule proximal and distal convoluted tubule in T1 compared to the T2 and T3 (P<0.05). The RT-PCR also confirmed this outcome. The results suggested that vitamin C consumption by the mother during the gestation period could ameliorate the nicotine effects on renal extracellular matrix of the offspring.