This study was conducted to investigate the effect of different levels of Protexin on feed efficiency and egg laying traits of Japanese quails. Forty eight Japanese quails (Coturnix japonica), having average age six weeks were equally allocated to four treatments each containing three replicates. In each replicate were included three female and one male bird. Birds received diets which were supplemented with 0, 0.250, 0.500, and 1.000 gm Protexin per kg diet as control, treatments 2, 3 and 4 respectively. Feed intake was measured daily and feed conversion ratio was calculated. After 63 days of experimental period, egg weight, albumen weight, yolk weight, and shell weight were measured. Feed intake decreased significantly in group 3, while FCR decreased significantly in all treatments. Egg weight, shell weight, albumin weight increased significantly in group 3. However, yolk weight decreased significantly in group 4. In conclusion, Protexin at the level of 0.500 gm/kg of feed improved the egg traits in Japanese quail.