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PRINT: ISSN 2221-1896
ONLINE : ISSN 2223-0343
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Genetic variation and gene flow among Tunisian sheep populations using
Random Amplified Polymorphic DNA (RAPD) markers |
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Haifa
El-Hentati1,2*, Mohamed Ben Hamouda1 and Ali
Chriki2
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1Regional
Center of Agricultural Research and Development on North-East, Po Box
122, 2090 Mornag, Institution of Agricultural Research and Higher
Education, Tunisia; 2Faculty of Sciences of Bizerta, 7021
Jarzouna, University of Carthage, Tunisia
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Abstract |
Random amplified polymorphic DNA (RAPD) was used
to infer the magnitude and pattern of genetic differentiation among
sheep populations
belonging to two native Tunisian breeds (the Barbarine and the Western
thin tail).
Eight primers generated 62 scorable bands,
of which 44 were polymorphic.
Genetic differentiation between populations within breeds was detected
based on the estimated FST index. The value of gene flow (Nm)
was
5.5361
and
3.5763 in Barbarine and Western thin tail breeds
respectively.
The diversity within populations (Hs) recorded demonstrates important
genetic variation within them. The values of
genetic differentiation coefficient (Gst) over all loci were 0.0828 and
0.1227 respectively between populations of
the Barbarine and Western thin tail breeds.
The
principal component analysis (PCA) did not show a clear differentiation
between populations within breeds.
The results show that studied sheep populations maintain a high genetic
diversity in the range of 0.21-0.23 and their genetic differentiation is
consistent with their geographic origin. It is therefore necessary to
preserve the purity of the Tunisian local breeds that are well adapted
to country’s bioclimatic conditions.
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Keywords:
Genetic variation; gene flow; RAPD; bioclimatic zones; Tunisian sheep |
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To cite this article:
El-Hentati
H, M Ben Hamouda and A Chriki,
2013.
Genetic variation and gene flow among Tunisian sheep populations using
Random Amplified Polymorphic DNA (RAPD) markers.
Res.
Opin. Anim. Vet. Sci., 3(7), 209-213. |
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