PRINT: ISSN 2221-1896
ONLINE : ISSN 2223-0343

Expression of functional leptin receptor (OB-Rb) mRNA in the sheep tissues
Abbas Abavisani 1*, Hesam Dehghani 1 and Morteza Zahedi2
1Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine and Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran; 2Department of Basic Sciences, Veterinary Faculty, Ferdowsi University of Mashhad, Mashhad, Iran

Leptin, a 167-amino acid hormone, is secreted mainly by fat tissue. Leptin signaling plays crucial roles on the regulation of metabolism and reproductive function through endocrine and probably paracrine mechanisms. So, detection of leptin receptor in various tissues is essential to an understanding of leptin physiology in sheep. The aim of the present study was to investigate expression pattern of functional ovine leptin receptor (OB-Rb) in adult tissues using reverse transcription and polymerase chain reaction analysis (RT-PCR). Ovine beta actin gene was chosen as an internal positive control as well as RNA purification marker. RT-PCR experiments confirmed the expression of functional leptin receptor mRNA in ovine tissues including fat, liver, rumen and lung, but not in kidney and hypophysis. Characterization of functional leptin receptor in different organs suggests that leptin has paracrine/ autocrine effects on many target organs as well as their physiology in sheep which should be more investigated in the future.

Keywords: Sheep; leptin receptor mRNA (OB-Rb); tissues, RT-PCR
To cite this article: Abavisani A, Dehghani H and Zahedi M, 2012. Expression of functional leptin receptor (OB-Rb) mRNA in the sheep tissues. Res. Opin. Anim. Vet. Sci., 2(7), 449-453.

Home  |  Archive  |  Instructions  |  Submission  |  Editorial Board  |  Sample Paper

All rights reserved ©