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Identification of single nucleotide polymorphisms of Hsp70 gene in a
commercial broiler strain
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Abdolalizadeh N, Noshary A* and Eila N |
Department
of Animal Science, Karaj Branch, Islamic Azad
University,
Karaj, Iran
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Abstract |
The aim
of this study was to identify single nucleotide polymorphisms of heat
shock protein (hsp70) at exon region in Ross 308 commercial broiler
strains during 6 to 7 week of age. Hsp70 coding region was amplified by
PCR technique using specific primers with 359bp. Quality was performed
by loading in an agarose gel and single nucleotide polymorphism was
identified by direct sequencing technique. The results of the sequences
were determined by using the Multi-Align and Chromas software's and
possible single nucleotide polymorphisms were detected among the
different samples. A comparison of the sequence of samples specified in
the Multi-Align showed that sequences of samples are different in four
specific loci. Sequence comparison of different samples by Multi-Align
revealed that mutations happened at G36, G44, G132 and G335 loci.
Sequence was compared with obtained data from NCBI database using NCBI
Blast-Multi-Align site. The results showed missing T and A at locus G36
and locus G44 respectively. Also the transition of G to A at locus G132
and C to G at locus G335 was observed. The frequency of A and G at G132
is 0.3438 and 0.6265 respectively, also the frequency of G and C at G335
was 0.3312 and 0.6687 respectively.
The
results show that there is polymorphism in hsp70 in Ross308 which can be
used as a tool in future breeding program.
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Keywords:
hsp70
gene; broilers Ross308; single nucleotide polymorphism |
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To cite this article:
Abdolalizadeh N, A Noshary and N Eila, 2015.
Identification of single nucleotide
polymorphisms of Hsp70 gene in a commercial broiler strain.
Res.
Opin.
Anim. Vet. Sci., 5(6): 265-269. |
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