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ONLINE : ISSN 2223-0343


Genetic differentiation and gene flow between four Tunisian sheep populations (Ovis aries) using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) analysis

Haifa El Hentati1,2*, Mohamed Ben Hamouda1 and Ali Chriki2

1Regional center of agricultural research and development on north-east, Po Box 122, 2090 Mornag, Institution of Agricultural research and higher education, Tunisia; 2Faculty of Sciences of Bizerta, 7021 Jarzouna, University of Carthage, Tunisia

 
Abstract

In this study, random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) analysis was used to assess the genetic difference and gene flow among four sheep populations belonging to two native Tunisian breeds (the Barbarine and the Western thin tail). A total of 96 samples were typed using eight RAPD primers. 62 bands were scored, of which 44 bands (70.97%) were polymorphic. The unweighed pair-group method with arithmetic average (UPGMA) and principal component analysis (PCA) did not show a clear differentiation between populations within breeds. The coefficient of gene differentiation (Gst) between populations was relatively low. Analysis of molecular variance (AMOVA) showed that there was a genetic variation of 28.79% (FCT= 0.2879; P<0.001) among the breeds, 4.10% among populations within breeds (FSC= 0.0576; P<0.001)) and 67.10% (FST= 0.3289; P<0.001) within populations. The level of gene migration between populations within breeds is quite high to homogenizing them.

Keywords: Genetic differentiation; RAPD-PCR; sheep; geographic areas
 
To cite this article: El Hentati H, M Ben Hamouda and A Chriki, 2013. Genetic differentiation and gene flow between four Tunisian sheep populations (Ovis aries) using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) analysis. Res. Opin. Anim. Vet. Sci., 3(6), 149-152.
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

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