E-ISSN 2223-0343

Apoptosis in ovine placentome in spontaneous and induced parturition using Aglepristone

Özalp RG and Zık B

1Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Uludag University, Görükle 16059 Bursa, Turkey; 2Department of Embryology and Histology, Faculty of Veterinary Medicine, Uludag University, Görükle 16059 Bursa, Turkey


The effects of Aglepristone treatment on placental expulsion in ewes were investigated. Fifteen crossbreed (Kivircik × Chios) pregnant does were randomly divided into three groups. Aglepristone was injected at the rate of 5 (AG5) and 10 mg/kg (AG10) once a day subcutaneously on day 140 and 141 of mating respectively.  Eves in control group (CG) were treated with 0.9% sodium chloride solution subcutaneously at the same day of pregnancy. Placentome samples were collected right after the expulsion of the last lamb. Expelled foetal membranes were weighed and expulsion period was recorded. Mean interval of expulsion of last lamb was not significant and the weight of expelled membrane was significant between AG5, AG10 and CG. In spite of non-significance of the expulsion interval between groups, the ewes in AG5 completed the placental expulsion beyond 10 hours. Therefore, tissue samples from ewes in AG5 were stained by TUNEL assay in order to assess the apoptosis. No significant difference in the number of apoptotic cells between control and Aglepristone groups was found (P>0.05). A few binucleated trophoblast cells (BNC) were detected in AG5. The results indicate that Aglepristone treatment at the rate of 5 mg/kg could prolong the time between expulsion of the last lamb and foetal membranes with reasonable number of apoptotic cells.

Keywords: Aglepristone; ewe; apoptosis; foetal membrane retention
To cite this article: Özalp RG and B Zık, 2015. Apoptotic status in ovine placentome both in Spontaneous parturition and induced parturition by a progesterone receptor blocker, aglepristone. Res. Opin. Anim. Vet. Sci., 5(5): 242-246.

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