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Apoptosis in ovine placentome in spontaneous and
induced parturition using Aglepristone
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Özalp RG and Zık B |
1Department of Obstetrics and Gynecology, Faculty of
Veterinary Medicine, Uludag University, Görükle 16059 Bursa, Turkey;
2Department of Embryology and Histology, Faculty of
Veterinary Medicine, Uludag University, Görükle 16059 Bursa, Turkey
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Abstract |
The
effects of Aglepristone treatment on placental expulsion in ewes were
investigated. Fifteen
crossbreed (Kivircik × Chios)
pregnant does were randomly divided into three groups. Aglepristone was
injected at the rate of 5 (AG5) and 10 mg/kg (AG10) once a day
subcutaneously on day 140 and 141 of mating respectively.
Eves in control group (CG) were
treated with 0.9% sodium chloride solution subcutaneously at the same
day of pregnancy. Placentome samples were collected right after the
expulsion of the last lamb. Expelled
foetal membranes were weighed and expulsion period was recorded. Mean
interval of expulsion of last lamb was not significant and the weight of
expelled membrane was significant between AG5, AG10 and CG. In spite of
non-significance of the expulsion interval between groups, the ewes in
AG5 completed the placental expulsion beyond 10 hours. Therefore, tissue
samples from ewes in AG5 were stained by TUNEL assay in order to assess
the apoptosis. No significant difference in the number of apoptotic
cells between control and Aglepristone groups was found (P>0.05). A few
binucleated trophoblast cells (BNC) were detected in AG5. The results
indicate that Aglepristone treatment at the rate of 5 mg/kg could
prolong the time between expulsion of the last lamb and foetal membranes
with reasonable number of apoptotic cells.
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Keywords:
Aglepristone; ewe;
apoptosis; foetal membrane retention |
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To cite this article:
Özalp RG and B Zık, 2015.
Apoptotic status in ovine placentome both in Spontaneous parturition and
induced parturition by a progesterone receptor blocker, aglepristone. Res. Opin. Anim. Vet. Sci., 5(5): 242-246. |
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