E-ISSN 2223-0343

Characterization of single nucleotide polymorphism in diacylglycerol acyltransferase (DGAT1) gene loci of Iranian Holstein Cattle

Sadegh Ahani1*, Mojtaba Hossienpour Mashhadi 1, Mohammad Reza Nassiri 2, Mehdi Aminafshar 3 and Mohammad Haddadi4

1Department of Animal Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran; 2Department of Animal Sciences, Ferdowsi University of Mashhad, Iran; 3Department of Animal Sciences, Islamic Azad University Research and Science, Tehran Branch, Iran; 4Department of Biology, Faculaty of Sicence, university of Zobol, Zabol, Iran

 
Abstract

Genetic improvements of beef and dairy cattle can bring significant advances in satisfying the global food demand expected to be doubled by 2050. Most of the traits in livestock are controlled by multiple genes so called as genetically complex traits. One of the bovine quantitative trait loci (QTL) that affects milk yield and composition was identified to be located on centromeric end of bovine chromosome 14. Diacylglycerol acyl transferase-1 (DGAT1) gene plays an important role in the synthesis and secretion of an enzyme that is able to catalyze the last step of triglyceride biosynthesis. A total of 100 Iranian Holstein dairy cattle blood samples were collected from Mashhad (Iran) dairy farms. Genomic DNA was extracted in a GuSCN-Silica Gel method. The considered fragment of 411 bps was amplified in a standard PCR reaction. The amplified fragments were digested in a PCR-RFLP method making use of Cfr1 restriction enzyme and subsequently were sequenced. Using PopGen analyzing software, genotype frequencies of KK, KA and AA genotypes were estimated to be 0.31, 0.41 and 0.28, respectively. The allele frequencies of K and A alleles were estimated as 0.515 and 0.485, respectively.

Keywords: DGAT1 gene, milk production trait, dairy milk, Holstein cattle
 
To cite this article: Ahani S, MH Mashhadi, MR Nassiri, M Aminafshar and M Haddadi, 2015. Characterization of single nucleotide polymorphism in diacylglycerol acyltransferase (DGAT1) gene loci of Iranian Holstein Cattle. Res. Opin. Anim. Vet. Sci., 5(5): 231-236.
 
 

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