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Characterization of single nucleotide polymorphism in
diacylglycerol acyltransferase (DGAT1) gene loci of
Iranian Holstein Cattle
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Sadegh
Ahani1*, Mojtaba Hossienpour Mashhadi
1, Mohammad Reza Nassiri 2,
Mehdi Aminafshar 3 and Mohammad Haddadi4 |
1Department of Animal Science, Mashhad Branch, Islamic
Azad University, Mashhad, Iran; 2Department of Animal
Sciences, Ferdowsi University of Mashhad, Iran; 3Department
of Animal Sciences, Islamic Azad University Research and Science, Tehran
Branch, Iran; 4Department of Biology, Faculaty of Sicence,
university of Zobol, Zabol, Iran
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Abstract |
Genetic improvements of beef
and dairy cattle can bring significant advances in satisfying the global
food demand expected to be doubled by 2050. Most of the traits in
livestock are controlled by multiple genes so called as genetically
complex traits. One of the bovine quantitative trait loci (QTL) that
affects milk yield and composition was identified to be located on
centromeric end of bovine chromosome 14. Diacylglycerol acyl
transferase-1 (DGAT1) gene plays an important role in the synthesis and
secretion of an enzyme that is able to catalyze the last step of
triglyceride biosynthesis. A total of 100 Iranian Holstein dairy cattle
blood samples were collected from Mashhad (Iran) dairy farms. Genomic
DNA was extracted in a GuSCN-Silica Gel method. The considered fragment
of 411 bps was amplified in a standard PCR reaction. The amplified
fragments were digested in a PCR-RFLP method making use of Cfr1
restriction enzyme and subsequently were sequenced. Using PopGen
analyzing software, genotype frequencies of KK, KA and AA genotypes were
estimated to be 0.31, 0.41 and 0.28, respectively. The allele
frequencies of K and A alleles were estimated as 0.515 and 0.485,
respectively.
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Keywords:
DGAT1 gene, milk
production trait, dairy milk, Holstein cattle |
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To cite this article:
Ahani S, MH Mashhadi, MR Nassiri, M Aminafshar and M Haddadi, 2015.
Characterization of single nucleotide polymorphism in
diacylglycerol acyltransferase (DGAT1) gene loci of
Iranian Holstein Cattle.
Res.
Opin. Anim. Vet. Sci., 5(5): 231-236. |
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