A 42 days study was conducted on 240 day-old Ross broiler chicks allocated randomly to four dietary treatments to compare the effect of different levels of creatine monohydrate (CrH) on blood (RBC count, PCV, Hb concentration, MCV, MCH and MCHC) and biochemical traits (creatinine and uric acid). Two types of diets were used, starter diet was used from one to 21 day and then grower diet was used till the end of the experiment. Beside control group, CMH was incorporated into the diet of broilers at the rate of 4 g/kg (T1), 8 g/kg (T2) and 12 g/kg (T3). The results showed that blood traits (RBC count, PCV, Hb concentration, MCV, MCH and MCHC) of birds of all supplemented groups (T1, T2 and T3) were not different (P<0.05) compared to control group. No significant difference was observed among all experimental groups concerning H/L ratio. Significant differences (P<0.05) was seen among all supplemented groups concerning biochemical blood traits (creatinine and uric acid). In conclusion, supplementation broiler ration with CrH did not affect the status of the bird at the used levels of CrM.