The study was carried out to determine the effect of photoperiod (PTP) on age and body weight at first ejaculation and on first and overall ejaculate characteristics of pubertal native toms. Thirty healthy grower native toms, 28 weeks old, were used for the study. The birds were assigned to one of 5 photoperiods or treatments (T): control or 12L:12D (T1), 14L:10D (T2), 16L:8D (T3), 18L:6D (T4) and 20L:4D (T5). Data included age and body weight at first ejaculation (AFE and BFE, respectively), first and subsequent ejaculate volumes (EV), sperm concentration (SPC), sperm motility (SPM), percent live sperm (LSP), dead sperm (DSP), normal sperm (NSP) and abnormal sperm (ASP). Analysis for effect of PTP on AFE and BFE and on first ejaculate characteristics involved initial body weight as a covariate. AFE for toms in 20L:4D was 36.95±0.43 wk compared to 41.59±0.46 wk for the control (P<0.05). BFE and weight gain up to first ejaculation did not vary with PTP. First ejaculate characteristics were affected by PTP except LSP, NSP and ASP. Effect of PTP on overall ejaculate characteristics was however, significant (P<0.05) for all traits with PTP 18L:6D and 20L:4D having the highest overall EV, SPC, LSP, SPM and NSP but least ASP and DSP. Significant (P<0.05) correlations existed between PTP, AFE and BFE and some semen quality traits in the first and overall ejaculates of the toms. It was concluded that photoperiod significantly affected the age at first ejaculation as well as first and subsequent ejaculate characteristics of native toms.