PRINT: ISSN 2221-1896
ONLINE : ISSN 2223-0343

Polymorphism of oxidized low density lipoprotein receptor1 (OLR1) gene in the Iranian Holstein dairy cattle

Mojtaba Hossienpour Mashhadi1*, Saeed Sobhanirad1, Seyed Eeman Nooraee1 and Reza Bahari Kashani1

Department of Animal Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran


The present study was carried out to determine the polymorphism in the 3' untranslated region of OLR1 gene in Iranian Holstein dairy Cattle. Oxidized low density lipoprotein receptor1 (LOX1) also known as oxidized low density lipoprotein receptor1 (OLR1) is a type II trans-membrane receptor which belongs to the C type lectin family. Polymorphism as well as significant associations was reported between single nucleotide polymorphism (SNP) in 3'untranslated region of OLR1 gene with milk production traits so OLR1 gene was chosen as a candidate gene. The blood samples of 153 dairy cattle from three different farms were used for extracting genomic DNA. A 146 bp fragment of 3'-untranslated region of OLR1 gene was amplified by standard polymerase chain reaction (PCR) procedure. Polymorphism of OLR1 gene was determined by PCR-RFLP technique. The number of animals with AA, AC and CC genotype were 32, 79 and 42 individuals respectively. The frequency of allele A and C was 0.4673 and 0.5327 respectively. The results of Chi square test showed that the population was under Hardy Weinberg equilibrium. The frequency of allele A and C is near 0.5 suggesting the effects of allele C on milk fat which may be increased in population.

Keywords: Dairy cattle; polymorphism, OLR1, Holstein
To cite this article: Mashhadi MH, S Sobhanirad, SE Nooraee and RB Kashani, 2014. Polymorphism of Oxidized Low density lipoprotein Receptor1 (OLR1) Gene in the Iranian Holstein dairy cattle. Res. Opin. Anim. Vet. Sci., 4(5): 237-240.

Home  |  Archive  |  Instructions  |  Submission  |  Editorial Board  |  Sample Paper

All rights reserved ©