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PRINT: ISSN 2221-1896
ONLINE : ISSN 2223-0343
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Polymorphism of oxidized low density lipoprotein receptor1 (OLR1) gene
in the Iranian Holstein dairy cattle
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Mojtaba
Hossienpour Mashhadi1*, Saeed Sobhanirad1, Seyed
Eeman Nooraee1 and Reza Bahari Kashani1
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Department of Animal
Science, Mashhad Branch, Islamic Azad University,
Mashhad,
Iran
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Abstract |
The present study was
carried out to determine the polymorphism in the 3'
untranslated region of OLR1 gene in Iranian Holstein dairy Cattle.
Oxidized low density lipoprotein receptor1 (LOX1) also known as oxidized
low density lipoprotein receptor1 (OLR1) is a type II trans-membrane
receptor which belongs to the C type lectin family. Polymorphism as well
as significant associations was reported between single nucleotide
polymorphism (SNP) in 3'untranslated region of OLR1 gene with
milk production traits so OLR1 gene was chosen as a candidate gene. The
blood samples of 153 dairy cattle from three different farms were used
for extracting genomic DNA. A 146 bp fragment of 3'-untranslated region
of OLR1 gene was amplified by standard polymerase chain reaction (PCR)
procedure. Polymorphism of OLR1 gene was determined by PCR-RFLP
technique. The number of animals with AA, AC and CC genotype were 32, 79
and 42 individuals respectively. The frequency of allele A and C was
0.4673 and 0.5327 respectively. The results of Chi square test showed
that the population was under Hardy Weinberg equilibrium. The frequency
of allele A and C is near 0.5 suggesting the effects of allele C on milk
fat which may be increased in population.
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Keywords:
Dairy cattle;
polymorphism, OLR1, Holstein |
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To cite this article:
Mashhadi MH, S Sobhanirad, SE Nooraee and
RB Kashani,
2014.
Polymorphism of Oxidized Low density lipoprotein
Receptor1 (OLR1) Gene in the Iranian Holstein dairy cattle.
Res.
Opin. Anim. Vet. Sci., 4(5): 237-240. |
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