E-ISSN 2223-0343

Bottleneck and molecular variance analyses in Senegalese local cattle breeds using microsatellite markers

Ndèye Penda Ndiaye1, 2*, Adama Sow2, Saliou Ndiaye3, Germain Jérôme Sawadogo2 and Mbacké Sembene1, 4

1Département de Biologie Animale, Faculté des Sciences et Techniques, Université Cheikh Anta Diop de Dakar (UCAD), BP 5005 Dakar Fann- Sénégal; 2Laboratoire d’endocrinologie et de radio-immunologie, Ecole Inter – Etats des Sciences et Médecine Vétérinaires (EISMV), BP 5077 Dakar Fann- Sénégal; 3Ecole Nationale Supérieure d’Agriculture (ENSA), Université de Thiès, BP A 296 Thiès RP,Sénégal; 4Laboratoire de Biologie des Populations Animales Sahélo-Soudaniennes, CBGP, Institut de Recherche pour le Développement (IRD), Bel Air, BP 1386 Dakar-Sénégal


The native Senegalese cattle were extensively used for crossbreeding purposes and breed development, which are assumed to improve livelihood of the most rural populations. This study was conducted in 4 native cattle breeds (Gobra zebu, Maure zebu, Djakoré and N’Dama) to assess the current genetic variation at molecular levels by using AMOVA tests and Bottleneck analysis. A total of 120 unrelated samples were collected from breeds reared in three agro-ecological areas of Senegal. In this study, 11 specific highly polymorphic microsatellite makers recommended by Food and Agriculture Organization (FAO) were used for animals genotyping. The basic measures of within breed variation and genetic differentiation were computed using bioinformatics’ software. All loci were polymorphic with a mean Polymorphic Information Content (PIC) of 0.76. The mean allelic richness per loci was 6.08. In average, high levels of heterozygosity was observed with a mean HO and HE of 0.71 and 0.79, respectively. The mean estimates of FIS over all loci were 0.073 indicating a significant heterozygosity deficiency relatively due to inbreeding among the cattle breeds or the occurrence of population substructure. Analysis of molecular variance (AMOVA) revealed that 4.76% of the total genetic variation was due to differences between populations (P<0.001), while the remaining 88.24% corresponded to differences within individuals (P<0.001). The qualitative test of mode shift analysis supported the conservative SMM model which indicated absence of genetic bottleneck in the recent past in Senegalese cattle populations. It was concluded from the current study of microsatellite markers that Senegalese local cattle populations was characterized by high level of genetic diversity and moderate genetic differentiation.

Keywords: AMOVA; Bottleneck; cattle; microsatellite markers; Senegal
To cite this article: Ndiaye NP, A Sow, S Ndiaye, GJ Sawadogo and M Sembène, 2015. Bottleneck and molecular variance analyses in Senegalese local cattle breeds using microsatellite markers. Res. Opin. Anim. Vet. Sci., 5(4): 158-164.

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