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Bottleneck and molecular variance analyses in Senegalese local cattle
breeds using microsatellite markers
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Ndèye Penda Ndiaye1, 2*, Adama Sow2,
Saliou Ndiaye3, Germain Jérôme Sawadogo2 and
Mbacké Sembene1, 4 |
1Département
de Biologie Animale, Faculté des Sciences et Techniques, Université
Cheikh Anta Diop de Dakar (UCAD),
BP 5005 Dakar Fann- Sénégal; 2Laboratoire d’endocrinologie et
de radio-immunologie, Ecole Inter – Etats des
Sciences et Médecine Vétérinaires
(EISMV), BP 5077 Dakar Fann- Sénégal; 3Ecole Nationale
Supérieure d’Agriculture (ENSA), Université de Thiès, BP A 296
Thiès RP,Sénégal; 4Laboratoire de Biologie des Populations
Animales Sahélo-Soudaniennes, CBGP, Institut de Recherche pour le
Développement (IRD), Bel Air, BP 1386 Dakar-Sénégal
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Abstract |
The native Senegalese cattle were extensively used for crossbreeding
purposes and breed development, which are assumed to improve livelihood
of the most rural populations. This study was conducted in 4 native
cattle breeds (Gobra zebu, Maure zebu, Djakoré and N’Dama) to assess the
current genetic variation at molecular levels by using AMOVA tests and
Bottleneck analysis. A total of 120 unrelated samples were collected
from breeds reared in three agro-ecological areas of Senegal. In this
study, 11 specific highly polymorphic microsatellite makers recommended
by Food and Agriculture Organization (FAO) were used for animals
genotyping. The basic measures of within breed variation and genetic
differentiation were computed using bioinformatics’ software. All loci
were polymorphic with a mean Polymorphic Information Content (PIC) of
0.76. The mean allelic richness per loci was 6.08. In average, high
levels of heterozygosity was observed with a mean HO and HE
of 0.71 and 0.79, respectively. The mean estimates of FIS
over all loci were 0.073 indicating a significant heterozygosity
deficiency relatively due to inbreeding among the cattle breeds or the
occurrence of population substructure. Analysis of molecular variance
(AMOVA) revealed that 4.76% of the total genetic variation was due to
differences between populations (P<0.001), while the remaining 88.24%
corresponded to differences within individuals (P<0.001). The
qualitative test of mode shift analysis supported the conservative SMM
model which indicated absence of genetic bottleneck in the recent past
in Senegalese cattle populations. It was concluded from the current
study of microsatellite markers that Senegalese local cattle populations
was characterized by high level of genetic diversity and moderate
genetic differentiation.
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Keywords:
AMOVA;
Bottleneck; cattle; microsatellite markers;
Senegal |
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To cite this article:
Ndiaye NP, A Sow, S Ndiaye, GJ Sawadogo and M
Sembène,
2015.
Bottleneck and molecular variance analyses in Senegalese local cattle
breeds using microsatellite markers.
Res.
Opin. Anim. Vet. Sci., 5(4): 158-164. |
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