Random amplified polymorphic DNA (RAPD) analysis for three breeds (Commercial broiler, Aseel and Karknath) of poultry using thirty three primers was conducted. A total of 198 bands (136 polymorphic) generated 96.96% polymorphism. Mean number of band per individual was 113.16, ranging from 3 to 11 types and polymorphic information content ranged 0.23 to 0.47. Jaccard’s similarity index based on RAPD revealed 49% average similarity with a range of 14 to 83%. The SAHN clustering based on UPGMA algorithms put accession in two groups, one with two genotypes and another with 10 accessions. The high within and between groups diversity indicated that breed (Aseel and Karknath) would be useful and may be utilized for breeding program.