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Chemical composition and SDS-PAGE analysis of radiated, extruded and
sprouted soybean
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Mehdi Moghaddam1, Parvin Shawrang2, Ali Asghar
Sadeghi1* and Mohammad Chamani1 |
1Department of Animal Science, Science and Research
Branch, Islamic Azad University, Tehran, Iran
2Nuclear Science and Technology Research Institute,
Atomic Energy Organization, Karaj, Iran
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Abstract |
The aim
of the present study was to examine the effects of gamma irradiation and
extrusion on chemical composition and protein subunit patterns of
soybean (SB), sprouted soybean (SSB) and extruded soybean (EBM) using
SDS-PAGE electrophoresis. Part of the
soybeans was germinated
in 3 days in a pollution-free environment. After extracting
fat,
samples were
poured
in
extraction solution and stored at
temperature of -20°C for electrophoresis.
Ash decreased significantly (P<0.05) in soybean meal
radiated with 45kGy. Ether extract (EE) was reduced significantly
(P<0.05) in soybean meal irradiated with 15kGy and sprouted soybean
irradiated with dose 15 kGy. Neutral detergent fibre (NDF) decreased
significantly (P<0.05) in soybean irradiated with dose 30 kGy and
soybean meal irradiated with dose 30 kGy. Acid detergent fibre (ADF)
decreased significantly (P<0.05) in soybean irradiated with dose 30 kGy
and 45 kGy. Acid detergent lignin (ADL) decreased significantly (P<0.05)
in soybean irradiated with dose 45 kGy and extruded sprouted soybean.
Regarding SDS-PAGE electrophoresis results, SB,
SSB and EBM proteins were mainly composed of
two major polypeptides including Glycinin and β-Conglycinin and the
subunit molecular weights (MWs) were affected by the processing. The results of the study showed that the extrusion
had the
greatest impact
on reducing
Glycinin subunits and
dose of 15 and 45 kGy
of the gamma irradiation
reduced and increased
MWs of
the protein
subunits, respectively. Gamma irradiation cross-linking the subunit of
proteins which this characterize can be a good substitute for SB, SSB
and EBM digestion and absorption
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Keywords:
Extruding; gamma;
molecular weights; sprout |
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To cite this article:
Moghaddam M, P Shawrang, AA Sadeghi and M Chamani, 2016.
Chemical composition and SDS-PAGE analysis of
radiated, extruded and sprouted soybean.
Res.
Opin.
Anim. Vet. Sci., 6(3): 101-107. |
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