E-ISSN 2223-0343

Chemical composition and SDS-PAGE analysis of radiated, extruded and sprouted soybean

Mehdi Moghaddam1, Parvin Shawrang2, Ali Asghar Sadeghi1* and Mohammad Chamani1

1Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
2Nuclear Science and Technology Research Institute, Atomic Energy Organization, Karaj, Iran

 
Abstract

The aim of the present study was to examine the effects of gamma irradiation and extrusion on chemical composition and protein subunit patterns of soybean (SB), sprouted soybean (SSB) and extruded soybean (EBM) using SDS-PAGE electrophoresis. Part of the soybeans was germinated in 3 days in a pollution-free environment. After extracting fat, samples were poured in extraction solution and stored at temperature of -20°C for electrophoresis. Ash decreased significantly (P<0.05) in soybean meal radiated with 45kGy. Ether extract (EE) was reduced significantly (P<0.05) in soybean meal irradiated with 15kGy and sprouted soybean irradiated with dose 15 kGy. Neutral detergent fibre (NDF) decreased significantly (P<0.05) in soybean irradiated with dose 30 kGy and soybean meal irradiated with dose 30 kGy. Acid detergent fibre (ADF) decreased significantly (P<0.05) in soybean irradiated with dose 30 kGy and 45 kGy. Acid detergent lignin (ADL) decreased significantly (P<0.05) in soybean irradiated with dose 45 kGy and extruded sprouted soybean. Regarding SDS-PAGE electrophoresis results, SB, SSB and EBM proteins were mainly composed of two major polypeptides including Glycinin and β-Conglycinin and the subunit molecular weights (MWs) were affected by the processing. The results of the study showed that the extrusion had the greatest impact on reducing Glycinin subunits and dose of 15 and 45 kGy of the gamma irradiation reduced and increased MWs of the protein subunits, respectively. Gamma irradiation cross-linking the subunit of proteins which this characterize can be a good substitute for SB, SSB and EBM digestion and absorption

Keywords: Extruding; gamma; molecular weights; sprout
 
To cite this article: Moghaddam M, P Shawrang, AA Sadeghi and M Chamani, 2016. Chemical composition and SDS-PAGE analysis of radiated, extruded and sprouted soybean. Res. Opin. Anim. Vet. Sci., 6(3): 101-107.
 
 
 
 

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