The success of an artificial insemination program in ostriches is highly dependent on the yield of viable semen. We, therefore, tested the effect of collection method (Massage, dummy or teaser) on semen quality of male ostriches. Male and female ostriches selected for training were from a breeding flock consisting of 90 adult ostriches (3 to 7 years old). Nine males were used in training for massage, dummy or teaser distributed equally. Semen characteristics were evaluated fortnightly for three consecutive months from all males involved in all semen collection methods. Semen quality traits involved in this study were ejaculate volume, sperm concentration, total number of spermatozoa, mass motility, individual motility and percentages of dead spermatozoa, abnormal spermatozoa and abnormal acrosome. Results revealed that using teaser female method, male ostriches recorded the highest values (P≤0.05) of ejaculated volume, spermatozoa concentration, total number of spermatozoa, mass motility, and individual motility and the lowest (P≤0.05) percentage of dead spermatozoa, abnormal spermatozoa and abnormal acrosome during the whole period of experiment while massage method recorded the lowest values for these traits. In conclusion, collecting semen during mating (teaser or dummy) appears to yield better quality of semen than manual massage.