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Molecular tracking of cultivable lactic acid
bacteria from digestive tract of Japanese quail (Coturnix coturnix japonica) fed with various grain sources
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Buyukunal EB1*, Bal MA2 and Hussein AJ1 |
1Department of Biology,
2Department of Animal Science, Kahramanmaras Sutcu Imam
University, 46100 Kahramanmaras, Turkey
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Abstract |
Lactic Acid Bacteria (LAB) possess many beneficial
functions in the digestive tract of animals. The aim of the present
study was to determine total LAB and Enterobacteriaceae counts and to
track cultivable LAB isolates from proventriculus and small intestines
of Japanese quails (Coturnix
coturnix japonica) fed with various grain sources. Three treatment
groups were fed with diets, designated as BAD, WAD or CAD, depending on
replacement of 50% (w/w) of basal or control diet (CD) with barley,
wheat and corn, respectively. The tracking of cultivable LAB isolates
(n=66) was performed based on Enterobacterial Repetitive Intergenic
Consensus PCR (ERIC-PCR) and Amplified Ribosomal DNA Restriction
Analysis (ARDRA) with combination of 16S rRNA gene sequencing. The
average LAB counts within proventriculus samples were the highest for
quails fed with WAD (5.47 cfu/g) and control diet (5.43 cfu/g). The
highest LAB counts were detected in small intestine samples for quails
fed with CAD (6.28 cfu/g). Although proventriculus and intestinal
samples of BAD fed animals had the lowest LAB count, they had the most
diverse LAB community in terms of number of species detected with
molecular tracking. Moreover, ERIC-PCR analysis and sequencing revealed
that Lactobacillus salivarius
was the most commonly detected species in both parts of digestive tract
of quails. Overall, feeding quails with different grain containing diets
affected both total number and diversity of LAB species within
proventriculus and small intestine samples. In addition, ERIC-PCR was
more powerful method than ARDRA to monitor diet dependent changes in
cultivable LAB composition within proventriculus and small intestine.
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Keywords:
ARDRA; digestive tract;
ERIC-PCR; Japanase quail; Lactic Acid Bacteria |
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To cite this article:
Buyukunal EB, Bal MA and Hussein AJ, 2017.
Molecular tracking of
cultivable Lactic Acid Bacteria from digestive tract of Japanese quail (Coturnix
coturnix japonica) fed with various grain sources.
Res.
Opin. Anim. Vet. Sci., 7(2): 33-41. |
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