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Live birth after transfer of vitrified embryos from
superovulated goats
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Marlon B. Ocampo1,3, Juliana Q. Silvestre2
,Virgilio D. Viernes3 and Lerma C. Ocampo1 |
1Reproductive Biotechnology Unit, Philippine Carabao
Center, Science City of Muñoz, Nueva Ecija, Philippines
2College of Veterinary Medicine, DMMMSU, Bacnotan, La
Union; 3College of Veterinary Science and Medicine, Central
Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines
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Abstract |
In this
study, the viability of vitrified goat embryos recovered from
superovulated non-descript Philippine goats were evaluated post-warming
by transferring to surrogate does. Of 17 does treated for superovulation,
3 did not respond and 14 responded with a 13.07 mean ovulation rate. An
average of 9.7 embryos per doe was collected at different developmental
stages. Using a container-less minimum drop size (MDS) method of
vitrification, 50 and 64 embryos were exposed to vitrification solution
1 (VS1) containing 10% ethylene glycol in basic medium (BM) consisting
of Hepes buffered TCM-199 medium + 20% estrus-doe serum solution for 3
min and 10 min, respectively. Immediately thereafter, the embryos were
transferred to 40% EG + 1 M sucrose in BM for 45 Sec before recovering
and dropping directly into LN2. After 3 months of storage,
the embryos were warmed in 2 ml of 0.3 M sucrose in BM for 5 min and
washed twice before transferring into culture medium. Morphologically
normal embryos were then transferred surgically to 6 recipients. Seven
kids were born after a normal gestation period with birthweights ranging
from 0.78–2.5 kg. The results highlight the usefulness of the MDS method
of vitrification using an ethylene glycol based solution in the
cryopreservation of goat embryos. That, continuous research effort
should be made for optimizing cryopreservation protocols for
conservation of animal genetic resources.
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Keywords:
Embryo transfer; live
birth; superovulation; vitrification |
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To cite this article:
Ocampo MB, JQ Silvestre,
VD Viernes and LC Ocampo, 2016.
Live birth after transfer of vitrified embryos from
superovulated goats.
Res.
Opin. Anim. Vet. Sci., 6(2): 47-52. |
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