This study was carried out to test the efficacy of colonic submucosal patches to repair gastric defect in dogs. Under general anesthesia, a five cm square patch of colonic submucosa was used to reconstruct an experimentally induced gastric defect in twelve local breed of dogs of both sexes. Clinically, the animals showed mild deterioration in general condition, loss of appetite and abdominal pain. These signs subsided gradually during the few days after the operation. No serious intraoperative and postoperative complications were encountered. On gross and laparoscopic examination, no distinguishable differences were detected between graft and recipient stomach and only mild adhesions were detected. Histopathological examination revealed excessive granulation tissue and fibrous connective tissue with mature collagen fibres after 60 and 90 days post-surgery, respectively. In conclusion, autologous colonic submucosal graft could be used for reconstruction of experimental gastric defect in dogs.