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Simple gene regulatory network of immune system
candidate genes in dairy cattle
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Mostafa Ghaderi-Zefrehei*1, Mustafa
Muhaghegh Dolatabady1 and EbrahimRowghani2 |
1Department of Animal
science, School of Agriculture, YasoujUnivesity, Yasouj,
Iran
2Department of Animal
sciences, College of Agriculture,
Darab
Branch,
Islamic Azad University, Darab, Iran
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Abstract |
Given a
sample space with many random variables defined on it, it makes lots of
scene to think of some kind of association among them. Generally, sample
space due to biological experiment is thought to create many random
variable with biologically motivated inter or intra-relationship. In
this study, our sample space contained mRNA profile of 6 immune system
candidate genes: Interfron Gamma (IFN-γ), Tomur Necrotic Factor alpha
(TNF-α), Granolocyt-Macrofage Colony Stimulating Factor (GM-CSF),
Interleukine-2 (IL-2), Interleukine-6 (IL-6) and Interleukine-8 (IL-8)
in which their transcriptome were obtained. These genes were due to
dairy cattle in different biological states e.g. the early, middle, late
of lactation and cows suffering from mastitis. Using Bayesian Network
(BN), we concluded that first, many genes showed independent behaviour
in terms of regulation (there were not any wired regulation among them
over different biological states) second, the mode of regulation changed
across different biological states. In mastitis state, IL-8 shown
to be regulator of TNF-a, IL-8 was regulator of GM-CSF
in early lactation, in middle of lactation, IL-2 shown to be
regulator of IFN- γ and in late of lactation IL-8 turned
out to be regulator of GM-CSF. This research revealed that the
mode of regulation of candidate genes was not identical over different
biological states. Overall, we showed that aforementioned immune system
candidate genes should be seen in the biological context which they are
functioning. Therefore, if the objective is to tackle with mastitis
using drug targeting studies or in genetic selection, it is more
relevant to pay close attention to IL-8 as it is predicted to
show the mode of regulator in mastitis state in dairy cattle. However,
this gene was also the regulator of other different genes in across
different biological states.
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Keywords:
Bayesian
network; immune system; dairy cattle; gene regulatory network |
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To cite this article:
Ghaderi-Zefrehei M, MM Dolatabady and E Rowghani, 2015.
Simple gene regulatory network of immune system
candidate genes in dairy cattle.
Res.
Opin.
Anim. Vet. Sci., 5(12): 499-506. |
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