Theileriosis is distributed worldwide and its prevalence leads to sever economical losses among farm animal. This study was conducted to determine Theileria species in goat. Blood samples from 400 randomly selected healthy goats were examined using parasitological method and the genomic DNA was extracted from blood and amplified using common primers targeting to 18S rRNA of Theileria spp, subsequently semi-nested PCR analyses with specific primers for Theileria lestoquardi ,T. annulata, and T. ovis. The result showed that 1.7% (7/400) cases were positive for the presence of Theileria spp. in microscopical examination, and 6% (24/400) positive for Theileria ovis in semi-nested PCR. The observation showed that there was no other DNA of protozoan parasites existed in the blood samples of goats in this region. It can be concluded that Theileria ovis is detectable in goats without any clinical manifestation.