E-ISSN 2223-0343

Molecular detection of Theileria ovis in goat in Tehran province, Iran

Mahdi Khodaveisi1, Sadegh Rahbari1*, Parviz Shayan2 and Nasser Hoghooghi Rad1

1Department of Parasitology, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran, 2Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran


Theileriosis is distributed worldwide and its prevalence leads to sever economical losses among farm animal. This study was conducted to determine Theileria species in goat. Blood samples from 400 randomly selected healthy goats were examined using parasitological method and the genomic DNA was extracted from blood and amplified using common primers targeting to 18S rRNA of Theileria spp, subsequently semi-nested PCR analyses with specific primers for Theileria lestoquardi ,T. annulata, and T. ovis. The result showed that 1.7% (7/400) cases were positive for the presence of Theileria spp. in microscopical examination, and 6% (24/400) positive for Theileria ovis in semi-nested PCR. The observation showed that there was no other DNA of protozoan parasites existed in the blood samples of goats in this region. It can be concluded that Theileria ovis is detectable in goats without any clinical manifestation.

Keywords: Theileria ovis; Semi-nested PCR; goat; Iran
To cite this article: Khodaveisi M, S Rahbari, P Shayan  and NH Rad, 2015. Molecular detection of Theileria ovis in goat in Tehran province, Iran. Res. Opin. Anim. Vet. Sci., 5(12): 489-493.

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