This study was conducted at three private herds around Sulaimania city on a total of 75 Karadi sheep of both sexes (18 ram and 57 ewes) randomly selected for blood sampling to establish mean values of some biochemical parameters as reference values for Karadi sheep. The serum biochemical parameters measured in the study included total cholesterol, high density lipoprotein (HDL), low density lipoprotein (LDL), very low density lipoprotein (VLDL), triglycerides, total protein (TP), serum albumin, serum globulin, calcium (Ca), phosphorus (P), magnesium (Mg), sodium (Na), potassium (K), chloride (Cl), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) activities. Results revealed that some serum biochemical values were within the reference range. However, higher concentration of LDL, VLDL, triglycerides, serum albumin, serum globulin and ALT activity in Karadi sheep were detected than those published earlier. However, lower concentration of TP was recorded when compared to references range.