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Molecular typing of infectious bursal disease virus field strains in
endemic settings of Africa
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Christopher Jacob Kasanga |
Department of Veterinary Microbiology and Parasitology, Sokoine
University of Agriculture, P.
O. Box 3019, Morogoro, Tanzania
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Abstract |
Detection and differentiation of serotype 1 infectious bursal disease
(IBD) virus (IBDV) field strains are important for effective control of
IBD. Restriction enzyme analyses have been reported to differentiate
serotype 1 strains into specific genotypes. However, the methods
previously reported are not suitable for genotyping the African very
virulent IBDV (VV-IBDV) in endemic settings of Africa. In this study, an
appropriate molecular typing method for IBDV field strains with
restriction endonucleases was determined. Six restriction patterns were
identified to categorize the serotype 1 strains into six molecular
groups by using Aat I, Ban III, Bsp MI, Ssp I, Sac I and Nco I
restriction endonucleases. One restriction pattern was specific for the
classical IBDV strains, whereas five patterns were able to categorize
the VV-IBDV strains into five molecular groups. Of the five restriction
patterns for VV-IBDV, one pattern was unique to the Tanzanian VV-IBDVs,
whereas four patterns were able to differentiate the African and the
European/Asian VV-IBDV genotypes. The six restriction endonucleases were
practically applied to differentiate the Tanzanian and Zambian field
IBDV strains into specific molecular groups. The molecular typing method
reported in this study could be used for epidemiological surveillance of
the field IBDV strains, particularly in areas where sequencing cannot be
performed.
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Keywords:
IBDV; molecular typing; restriction endonucleases; Africa |
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To cite this article:
Kasanga CJ,
2015.
Molecular typing of infectious bursal disease virus field strains in
endemic settings of Africa.
Res.
Opin. Anim. Vet. Sci., 5(11): 429-434. |
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