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Potentiating effects of Lactuca serriola on pentobarbital-induced sleep

Azar Hosseini1, Ahmad Ghorbani1, Hamid Reza Sadeghnia1,2,3, Arezoo Rajabian3 and Hassan Rakhshandeh1,3*

1Pharmacological  Research  Center  of  Medicinal  Plants,  School  of  Medicine,  Mashhad; University of Medical Sciences, Mashhad, Iran; 2Neurocognitive Research Center, School of Mashhad University of Medical Sciences, Mashhad, Iran, Iran; 3Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran


Insomnia is the most common sleep disorder. The present study was carried out to evaluate the sedative-hypnotic effect of hydro-alcoholic extract (HAE) of lettuce and its water fraction (WF), ethyl acetate fraction (EAF) and n-butanol fraction (NBF). Extract and its fractions were injected to mice 30 min before the pentobarbital. Additionally, the effects of flumazenil on the hypnotic activity of the extract and its toxic effect were determined in vivo and in vitro situations. The HAE prolonged the pentobarbital-induced sleep duration at doses of 50-400 mg/kg (50 mg/kg; P<0.05, 100 and 200 mg/kg; P<0.01). In comparison to the control group, HAE at dose of 400 mg/kg increased sleeping time more than diazepam (P<0.001 vs control). Also flumazenil (2 mg/kg) reversed the effects of both diazepam (P<0.001 vs diazepam group) and 400 mg/kg of HAE (P<0.001 vs HAE group). The NBF was the only fraction which could increase sleep duration (P<0.001) and decrease sleep latency (P<0.001). Flumazenil reversed hypnotic effect of the extract and diazepam. The LD50-value for HAE was found to be 4.8 g/Kg. HAE and its fractions did not show any neurotoxic effect in cultured PC12-cell line. HPLC profile of the extract showed five major peaks with different retention times. The results suggested that Lactuca serriola potentiated pentobarbital hypnosis without major toxic effect. Most probably, the main component (s) responsible for this effect was most likely to be non-polar agent (s) which is found in NBF of this plant.

Keywords: Insomnia; diazepam; Lactuca serriola; sleep; PC12
To cite this article: Hosseini A, A Ghorbani, HR Sadeghnia, A Rajabian and H Rakhshandeh, 2014. Potentiating effects of Lactuca serriola on pentobarbital-induced sleep. Res. Opin. Anim. Vet. Sci., 4(11): 601-607.

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