|
PRINT: ISSN 2221-1896
ONLINE : ISSN 2223-0343
|
|
|
Potentiating effects of Lactuca serriola on
pentobarbital-induced sleep
|
Azar Hosseini1, Ahmad Ghorbani1,
Hamid Reza Sadeghnia1,2,3, Arezoo Rajabian3 and
Hassan Rakhshandeh1,3* |
1Pharmacological
Research Center
of Medicinal
Plants, School
of Medicine,
Mashhad; University of Medical Sciences, Mashhad, Iran; 2Neurocognitive
Research Center, School of Mashhad University of Medical Sciences,
Mashhad, Iran, Iran; 3Department of Pharmacology, School of
Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
|
|
Abstract |
Insomnia is the most
common sleep disorder. The present study was carried out to evaluate the
sedative-hypnotic effect of hydro-alcoholic extract (HAE) of lettuce and
its water fraction (WF), ethyl acetate fraction (EAF) and n-butanol
fraction (NBF). Extract and its fractions were injected to mice 30 min
before the pentobarbital. Additionally, the effects of flumazenil on the
hypnotic activity of the extract and its toxic effect were determined
in vivo and in vitro
situations. The HAE prolonged the pentobarbital-induced sleep duration
at doses of 50-400 mg/kg (50 mg/kg; P<0.05, 100 and 200 mg/kg; P<0.01).
In comparison to the control group,
HAE at dose of 400 mg/kg
increased sleeping time more than diazepam (P<0.001 vs control).
Also flumazenil (2 mg/kg) reversed the effects of both diazepam (P<0.001
vs diazepam group) and 400 mg/kg of
HAE (P<0.001 vs HAE
group). The NBF was the only fraction which could increase sleep
duration (P<0.001) and decrease sleep latency (P<0.001). Flumazenil
reversed hypnotic effect of the extract and diazepam. The LD50-value
for HAE was found to be 4.8 g/Kg. HAE and its fractions did not show any
neurotoxic effect in cultured PC12-cell line. HPLC profile of the
extract showed five major peaks with different retention times. The
results suggested that Lactuca serriola potentiated pentobarbital
hypnosis without major toxic effect. Most probably, the main component
(s) responsible for this effect was most likely to be non-polar agent
(s) which is found in NBF of this plant.
|
Keywords:
Insomnia; diazepam;
Lactuca serriola; sleep; PC12 |
|
To cite this article:
Hosseini A, A Ghorbani,
HR Sadeghnia, A Rajabian and H Rakhshandeh, 2014.
Potentiating effects of Lactuca serriola on pentobarbital-induced
sleep.
Res.
Opin. Anim. Vet. Sci., 4(11): 601-607. |
|
|