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PRINT: ISSN 2221-1896
ONLINE : ISSN 2223-0343
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Modifications in serum Amyloid A and Haptoglobin in mice following oral
inoculation of graded doses of P. multocida type B: 2 and its
lipopolysaccharide
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*Faez Firdaus Jesse Abdullah1, 2, Omar Suwaidan Ali1,
Lawan Adamu1, 4, Yusuf Abba3,4, Hazilawati binti
Hamzah3, Mohd-Azmi M. L3, Abdul Wahid Haron1,
2 and Mohd Zamri-Saad3 |
1Department
of Veterinary Clinical Studies, 2Research Centre for Ruminant
Disease, 3Department of Veterinary Pathology and
Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia,
43400 UPM Serdang, Selangor, Malaysia; 4Faculty of Veterinary
Medicine, University of Maiduguri, PMB 1069, Borno State, Nigeria
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Abstract |
This study was conducted to investigate the serum Amyloid A (SAA) and
Haptoglobin (Hp) in the mice infected with Pasteurella multocida
type B: 2 and its lipopolysacheride (LPS) inoculated through the oral
route with graded doses (101, 103, 105,
107 and 109 cfu). Sixty healthy Balb c mice were
placed in twelve plastic cages each one containing five mice. The mice
were divided into three major groups (A, B and C). Group A is the
control group (n = 10) and these were inoculated with 0.4 ml of PBS (pH
7.4) orally. The treatment groups (B; n = 25 and C; n = 25) were
inoculated with P. multocida type B: 2 and its LPS respectively.
The mice in group B and C were further divided into five subgroups. The
subgroups were designated based on the graded doses as B101,
B103, B105, B107 and B109
for Pasteurella multocida and C101, C103,
C105, C107 and C109 for LPS. The mice
were observed for 120 hours post-inoculation. The concentration of Hp
was significantly higher (P<0.05) in the B109 cfu of P.
multocida type B: 2 and LPS compared to the control group and the
other treatments. In conclusion, Pasteurella multocida type B: 2
and its LPS are able to increase Hp in mice during acute infections.
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Keywords:
Lipopolysaccharides; Pasteurella multocida type B2; Acute phase
proteins; graded doses; oral route; mice |
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To cite this article:
Abdullah FFJ, OS Ali, L Adamu, Y Abba, HB Hamzah, ML Mohd-Azmi, AW Haron
and M Zamri-Saad,
2014.
Modifications in serum Amyloid A and haptoglobin in mice following oral
inoculationof graded doses of P. multocida type B: 2 and its
lipopolysaccharide.
Res.
Opin. Anim. Vet. Sci., 4(11): 587-592. |
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