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Neuroprotective effects of ganoderic acid extract against epilepsy in primary hippocampal neurons |
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Junxing Liu1,
Xiaoguang Liu1, Lin Wang2*,
Fei Wu1 and Zhiwei Yang1*
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1School
of Basic Medical Sciences, Jiamusi University, Jiamusi 154007, China;
2The First Affiliated Hospital of Jiamusi University, Jiamusi
154003, China
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Abstract |
The purpose of this study was to investigate the
effects of
ganoderic acid extract
(GAE) on magnesium-free induced epilepsy in primary hippocampal neurons.
Neuroprotective effects of GAE were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay,
flow cytometry as well as real time PCR (RT-PCR). GAE improved the cell
viability of hippocampal neurons in the dose and time-dependent
relationships, the best optimal dose is 200 μg/mL and action time 24
hours. Treatment of hippocampal neurons with GAE in the presence of
magnesium-free significantly reduced the number of apoptotic cells, and
decreased the expressions of
Caspase-3 and
PDCD5 mRNA. Collectively, our results showed that the
neuroprotective potential of GAE against
magnesium-free induced epilepsy in primary hippocampal
neurons.
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Keywords:
Neuroprotective;
Ganoderic
acid extract; Hippocampal
neurons; Epilepsy |
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To cite this article:
Liu J, X Liu, L Wang, F Wu and Z Yang, 2013.
Neuroprotective effects of ganoderic acid extract against epilepsy in
primary hippocampal neurons.
Res.
Opin. Anim. Vet. Sci., 3(11), 420-425. |
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