E-ISSN 2223-0343


Neuroprotective effects of ganoderic acid extract against epilepsy in primary hippocampal neurons

Junxing Liu1, Xiaoguang Liu1, Lin Wang2*, Fei Wu1 and Zhiwei Yang1*

1School of Basic Medical Sciences, Jiamusi University, Jiamusi 154007, China; 2The First Affiliated Hospital of Jiamusi University, Jiamusi 154003, China

 
Abstract

The purpose of this study was to investigate the effects of ganoderic acid extract (GAE) on magnesium-free induced epilepsy in primary hippocampal neurons. Neuroprotective effects of GAE were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry as well as real time PCR (RT-PCR). GAE improved the cell viability of hippocampal neurons in the dose and time-dependent relationships, the best optimal dose is 200 μg/mL and action time 24 hours. Treatment of hippocampal neurons with GAE in the presence of magnesium-free significantly reduced the number of apoptotic cells, and decreased the expressions of Caspase-3 and PDCD5 mRNA. Collectively, our results showed that the neuroprotective potential of GAE against magnesium-free induced epilepsy in primary hippocampal neurons.

Keywords: Neuroprotective; Ganoderic acid extract; Hippocampal neurons; Epilepsy
 
To cite this article: Liu J, X Liu, L Wang, F Wu and Z Yang, 2013. Neuroprotective effects of ganoderic acid extract against epilepsy in primary hippocampal neurons. Res. Opin. Anim. Vet. Sci., 3(11), 420-425.
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

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