E-ISSN 2223-0343


Molecular detection of Bovine Leukemia Virus (BLV) in the frozen semen samples of bulls used for artificial insemination in Iran

Faham Khamesipour1,3*, Abbas Doosti2, Amin Khodadoustan Shahraki3 and Mehdi Goodarzi4

1Young Researchers and Elite Club, Islamic Azad University- Shahrekord Branch, Shahrekord, Iran
2Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
3 Under Graduated Student of Veterinary Medicine, College of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran; 4Department of Clinical Sciences, School of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran

 
Abstract

Bovine Leukemia Virus (BLV) is a causative agent of bovine leukosis responsible for lymphoproliferative disorders in cattle. The aim of this study was to detect the BLV in the frozen semen samples of Iranian bulls used for artificial insemination using PCR. A total of 45 frozen Semen samples were obtained from 45 bulls used for artificial insemination centres. DNA was extracted and 385 bp fragment of the gag gene of the virus was visualized in a 1% agarose gel electrophoresis. Viral DNA was detected in nine of 45 (20%) frozen semen samples of bull’s that were used for artificial insemination. The results of this study demonstrated the high prevalence of BLV DNA in frozen semen samples of bulls. According to this finding it is clear that testing, segregation and culling programs can be effective in the control and prevent the bovine leukosis disease caused by BLV. Furthermore, examination of cattle semen samples that are used for artificial insemination is necessary to prevent the spread of BLV in this region.

Keywords: Artificial insemination; Bovine leukemia virus; bulls; PCR, semen
 
To cite this article: Khamesipour F, A Doosti, AK Shahraki and M Goodarzi, 2013. Molecular detection of Bovine Leukemia Virus (BLV) in the frozen semen samples of bulls used for artificial insemination in Iran. Res. Opin. Anim. Vet. Sci., 3(11), 412-416.
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

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