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Molecular detection of Bovine
Leukemia Virus (BLV) in the frozen semen samples of bulls
used for artificial insemination in Iran |
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Faham Khamesipour1,3*, Abbas Doosti2,
Amin Khodadoustan Shahraki3 and Mehdi Goodarzi4
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1Young Researchers and Elite Club, Islamic Azad
University- Shahrekord Branch,
Shahrekord,
Iran
2Biotechnology Research
Center, Islamic
Azad
University, Shahrekord Branch, Shahrekord,
Iran
3
Under Graduated Student of Veterinary Medicine,
College of Veterinary Medicine, Islamic Azad University, Shahrekord
Branch, Shahrekord, Iran; 4Department of Clinical Sciences,
School of Veterinary Medicine, Islamic Azad University, Shahrekord
Branch, Shahrekord, Iran
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Abstract |
Bovine Leukemia Virus (BLV) is a
causative agent of bovine leukosis responsible for lymphoproliferative
disorders in cattle. The aim of this study was to detect the
BLV in the frozen semen
samples of Iranian bulls used for artificial insemination using PCR. A
total of 45 frozen Semen samples were obtained from 45 bulls used for
artificial insemination centres. DNA was extracted and 385 bp fragment
of the gag gene of the virus was visualized in a 1% agarose gel
electrophoresis. Viral DNA was detected in nine of 45 (20%) frozen semen
samples of bull’s that were used for artificial insemination. The
results of this study demonstrated the high prevalence of
BLV DNA in frozen semen
samples of bulls. According to this finding it is clear that testing,
segregation and culling programs can be effective in the control and
prevent the bovine leukosis disease caused by BLV.
Furthermore, examination of cattle semen samples that are used for
artificial insemination is necessary to prevent the spread of BLV
in this region.
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Keywords:
Artificial insemination;
Bovine leukemia virus; bulls; PCR, semen |
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To cite this article:
Khamesipour F, A Doosti,
AK Shahraki and M Goodarzi, 2013.
Molecular detection of
Bovine Leukemia Virus (BLV)
in the frozen semen samples of bulls used for artificial insemination in Iran.
Res.
Opin. Anim. Vet. Sci., 3(11), 412-416. |
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