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Liquid chromatography method validation for the
simultaneous detection of
deoxynivalenol, zearalenone, T-2 and HT-2
mycotoxins in vegetal products |
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Miliţă N.M.1, Chifiriuc M.C.2*, Georgescu M.I.1,
Negreanu C.N.1, Feier S.1, Stroie O.1,
Ştefan V.1 and
Mihăescu G.2
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1
Sanitary Veterinary and Food Safety Directorate
Bucharest, Romania;
2Department of Botanics and Microbiology, Faculty of
Biology, Bucharest University, Bucharest, Romania
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| Abstract |
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Fusarium, Trichoderma, Stachybotrys and other fungal species are plant pathogens
usually associated with cereals, which under certain environmental
conditions can produce several secondary toxic metabolites. The major
toxins found in cereals and
cereal-based products such as deoxynivalenol, zearalenone, T-2 toxin and
HT-2 toxin can be harmful to human and animal health. Liquid
chromatography-tandem mass spectrometry (LC MS/MS) is a rapid and
sensitive analytical method used for the detection of mycotoxins
in vegetal products (cereals and
cereal-based products). Here, we report the validation parameters of the
LC MS/MS method,
for the simultaneous quantitative assay of
deoxynivalenol, zearalenone, T-2
toxin and HT-2 toxin accordingly to European Legislations concerning the
maximal permitted levels and detection methods for mycotoxins.
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Keywords:
liquid chromatography; mass spectrometry; deoxynivalenol; zearalenone;
T-2 toxin and HT-2 toxin |
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To cite this article:
Manuela MN, C Carmen, GM
Ioana, CN Negreanu, F Serin, S Oana, Ş Valentina and G Mihăescu, 2013.
Liquid
chromatography method validation for the simultaneous detection of
deoxynivalenol, zearalenone,
T-2 and HT-2
mycotoxins in vegetal products.
Res.
Opin.
Anim. Vet. Sci., 3(11), 391-394. |
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