This study was designed to investigate the effect of sumac (Rhus coriaria L.) and ajwain (Trachyspermum copticum) on performance of broiler chicks. A total 240 one day broiler chicks with an average weight of 38±3 g were randomly divided into four treatments. Each treatment was further divided into four replicates. Chicks were fed a basal diet (control group), basal diet + 0.02% sumac powder (T1), basal diet + 0.02% ajwain powder (T2) and basal diet + 0.02% virginiamycin powder (T3). At the end of trial (42 days), two birds form each treatment were weighed and slaughtered. Data showed that feed intake (FI) increased significantly in treated groups in comparison to control. Body weight gain and total body weight were also significantly higher in treated groups. Triglyceride and cholesterol level decreased significantly in T1 and T2 group. LDL level decreased significantly in T1 and HDL levels increased in the same group. Antibody titre increased significantly in T2 and T3 compared to the control group. Data on microbial population investigation showed that E. coli population decreased and lactobacillus increased significantly in treated groups. We concluded that the use of sumac and ajwain at the present level had almost similar beneficial effects on body performance, cholesterol profile and antibody titre in broiler chicks.