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PRINT: ISSN 2221-1896
ONLINE : ISSN 2223-0343
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Biochemical changes in muscle and gill tissues of rainbow trout treated
with various concentrations of pollen extract
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Sevgi
Durna Dastan1*, Taner Dastan2, Mehmet Fuat Gulhan3,
Ayse Kirkbes4 and Zeliha Selamoglu Talas4 |
1Department
of Zootechnical and Animal Nutrition,
Faculty of Veterinary, Cumhuriyet University,
58140, Sivas,
Turkey;
2Department
of Chemistry, Faculty of Arts and Sciences, Firat University, 23119,
Elazig, Turkey;
3Department of Medicinal and Aromatic Plants,
Vocational School of Technical Sciences, Aksaray University, Aksaray,
Turkey;
4Department of Biology, Faculty of Arts and Science,
NigdeUniversity, Nigde, Turkey
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Abstract |
The aim of this study was
to determine the effectiveness of pollen extract on biochemical
parameters in muscle and gill tissues of rainbow trout. Various
concentrations of the pollen extract (0.5, 2.5, 5, 10, 20 and 30 ppm)
for 96 h was applied on muscle and gills of
forty
nine rainbow trout (Oncorhynchus
mykiss). The malondialdehyde (MDA), total antioxidant status (TAS),
total oxidant status (TOS), oxidative stress index (OSI), total free
sulfhydryl groups were determined. MDA level decreased (P<0.05)
at the dose rate of 10-30 ppm concentration in muscle while in gills the
level of 10 ppm was the most effective. The dose rate of 10 ppm was
effective in TAS, TOS and OSI
for muscle and gill tissues. The highest value of total free
sulfhydryl groups was found at the dose rare of 5 and
10 ppm in muscle and tissues respectively. It is concluded that
pollen extract on muscle and gill tissues of fish is effective in
prevention of oxidative stress.
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Keywords:
Antioxidant; gill;
muscle; pollen; rainbow trout |
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To cite this article:
Dastan SD, T Dastan, MF Gulhan, A
Kirkbes and ZS Talas, 2014.
Biochemical changes in
muscle and gill tissues of rainbow trout treated with various
concentrations of pollen extract.
Res.
Opin. Anim. Vet. Sci., 4(10): 540-544. |
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