|
PRINT: ISSN 2221-1896
ONLINE : ISSN 2223-0343
|
|
|
Effect of dose dependent oral inoculation of Pasteurella multocida
type B: 2 in mice: Molecular detection and histopathological
evaluation
|
*Faez
Firdaus Jesse Abdullah1,3, Lawan Adamu1,4, Yusuf
Abba2,4, Abdulnasir Tijjani2,4 Konto Mohammed1,4,
Abdulrahman Omar2 and Abdul Aziz Saharee1,3 |
1Department of Veterinary Clinical Studies; 2Department
of Veterinary Pathology and Microbiology; 3Research Centre
for Ruminant Disease, Faculty of Veterinary Medicine, University Putra
Malaysia, 43400 UPM Serdang, Selangor, Malaysia; 4Faculty of
Veterinary Medicine, University of Maiduguri, P.M.B 1069, Borno State,
Nigeria
|
|
Abstract |
Haemorrhagic septicaemia
(HS) is an important disease of cattle and buffaloes worldwide, caused
by Pasteurella multocida type B: 2 and type E. Here, we evaluated
the clinical signs, gross and histopathological changes as well as
molecular detection of P. multocida in various organs of mice
following oral inoculation with graded doses of the bacterium. A total
of 32 healthy mice were divided into four groups of 8 mice each where
each group was inoculated orally with 0.4ml of P. multocida type
B: 2 at the dose of 10³ (Group 1), 105 (Group 2) and 107
(Group 3) colony forming unit (CFU) respectively, while Group 4 served
as a control. Post-mortem examination was conducted and gross lesions
were observed. Tissue samples were collected for molecular detection and
histo-pathological processing. All mice from the challenged groups
showed almost no significant (P>0.05) clinical signs. Evidently, the
severity of histo-pathological lesions in various organs was
significantly high in groups 2 and 3. Through polymerase chain reaction
detection (PCR), P. mulltocida type B: 2 was detected in all the
experimental groups. Overall, the oral inoculation of mice at different
doses of P. multocida type B: 2 results in clinical signs and
histopathological lesions of haemorrhagic septicaemia infection.
|
Keywords:
Haemorrhagic septicaemia;
Pasteurella multocida type B; mice;
histopathology; lesions; polymerase chain reaction (PCR); dose dependent |
|
To cite this article:
Abdullah FFJ, L Adamu, Y
Abba, A Tijjani, K Mohammed, A Omar and AA Saharee, 2014.
Effect of dose dependent
oral inoculation of Pasteurella multocida type B: 2 in mice:
Molecular detection and histopathological evaluation.
Res.
Opin.
Anim. Vet. Sci., 4(10): 535-539. |
|
|