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ONLINE : ISSN 2223-0343

Effect of dose dependent oral inoculation of Pasteurella multocida type B: 2 in mice: Molecular detection and histopathological evaluation

*Faez Firdaus Jesse Abdullah1,3, Lawan Adamu1,4, Yusuf Abba2,4, Abdulnasir Tijjani2,4 Konto Mohammed1,4,  Abdulrahman Omar2 and Abdul Aziz Saharee1,3

1Department of Veterinary Clinical Studies; 2Department of Veterinary Pathology and Microbiology; 3Research Centre for Ruminant Disease, Faculty of Veterinary Medicine, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; 4Faculty of Veterinary Medicine, University of Maiduguri, P.M.B 1069, Borno State, Nigeria

 
Abstract

Haemorrhagic septicaemia (HS) is an important disease of cattle and buffaloes worldwide, caused by Pasteurella multocida type B: 2 and type E. Here, we evaluated the clinical signs, gross and histopathological changes as well as molecular detection of P. multocida in various organs of mice following oral inoculation with graded doses of the bacterium. A total of 32 healthy mice were divided into four groups of 8 mice each where each group was inoculated orally with 0.4ml of P. multocida type B: 2 at the dose of 10³ (Group 1), 105 (Group 2) and 107 (Group 3) colony forming unit (CFU) respectively, while Group 4 served as a control. Post-mortem examination was conducted and gross lesions were observed. Tissue samples were collected for molecular detection and histo-pathological processing. All mice from the challenged groups showed almost no significant (P>0.05) clinical signs. Evidently, the severity of histo-pathological lesions in various organs was significantly high in groups 2 and 3. Through polymerase chain reaction detection (PCR), P. mulltocida type B: 2 was detected in all the experimental groups. Overall, the oral inoculation of mice at different doses of P. multocida type B: 2 results in clinical signs and histopathological lesions of haemorrhagic septicaemia infection.

Keywords: Haemorrhagic septicaemia; Pasteurella multocida type B;  mice; histopathology; lesions; polymerase chain reaction (PCR); dose dependent
 
To cite this article: Abdullah FFJ, L Adamu, Y Abba, A Tijjani, K Mohammed, A Omar and AA Saharee, 2014. Effect of dose dependent oral inoculation of Pasteurella multocida type B: 2 in mice: Molecular detection and histopathological evaluation. Res. Opin. Anim. Vet. Sci., 4(10): 535-539.
 
 

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