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Effect of buffers and egg yolk
concentrations on chilled and frozen-thawed Boer goat spermatozoa |
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Akeel Ahmed Memon1,3*, H.Wahid1*,
Y. Rosnina1, Y.M. Goh2 and M. Ebrahimi2
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1Department of Veterinary
Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra
Malaysia, 43400 UPM, Serdang, Selangor Darul Ehsan, Malaysia; 2Department
of Veterinary Preclinical Sciences, Faculty of Veterinary Medicine,
Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor Darul Ehsan,
Malaysia; 3Department of Animal Reproduction, Faculty of
Animal Husbandry and Veterinary Sciences, Sindh agriculture University
Tandojam Pakistan
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Abstract |
This experiment was carried out with the aim to compare
the effect of buffers and egg yolk concentrations on chilled and
frozen-thawed spermatozoa characteristics of Boer goat.
Semen was collected using an artificial vagina. After collection and
evaluation, ejaculates qualifying the standard criteria were pooled and
selected for extension and freezing. In experiment I, pooled semen was
split into three parts and each part was mixed with Tris, citrate and
skim milk based extender in two step dilution method and cooled at 4°C
for 2.5 h. It was observed that chilled spermatozoa in Tris citric acid
fructose egg yolk and skim milk buffers provided significantly (P<0.05)
better results in terms of motility, membrane integrity, normal
spermatozoa and viability as compared to citrate egg yolk buffer.
Furthermore, Numerically, Tris was superior in all sperm
parameters than the skim milk but the difference was non-significant
(P>0.05). Significant (P<0.05) improvement was observed with Tris
followed by skim milk and citrate buffers after freezing. This was
observed by significant improvement of motility and acrosome integrity
compared to citrate and skim milk. Furthermore, Tris, skim milk and
citrate provided the best results in terms of membrane integrity and
viability respectively. In experiment II, pooled semen was split into
three parts and each part was mixed with Tris based extender containing
different concentrations of egg yolk. The highest egg yolk level at 18%
improved (P<0.05) chilled frozen thaw spermatozoa quality of Boer goat.
Significantly better results (P<0.05) were also observed in all sperm
characteristics at 18% egg yolk after freezing. In conclusion, Tris
citric acid fructose with 18% egg yolk concentration can be used to
improve the chilled and frozen-thaw spermatozoa quality of Boer goat.
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Keywords:
Goat;
semen; freezing; extender |
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To cite this article:
Memon AA,
H Wahid, Y Rosnina, YM Goh and M Ebrahimi, 2013.
Effect of Buffers and Egg Yolk
Concentrations on Chilled and Frozen-Thawed Boer goat Spermatozoa.
Res.
Opin. Anim. Vet. Sci., 3(10), 374-379. |
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