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Polymorphism of stearoyl-coenzyme A desaturase 1(SCD1) gene in Iranian
holstein dairy cattle |
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Mojtaba
Hossienpour Mashhadi1*,
Saeed Sobhanirad1, Seyed Eeman Nooraee1 and Reza
Bahari Kashani1
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Department of Animal
Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran
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Abstract |
The aim of this study was
to investigate the polymorphism of SCD1 gene in
Holstein
dairy cattle. The identification of alleles of loci associated with
important function in animal metabolism could assist in recognizing
potential molecular marker for dairy cattle milk production traits. Milk
is essential for energy, protein, minerals and vitamins for the young
stock in the early phase of their life. The SCD1 gene, expressed in
adiposities mammary glands and also in other tissues, encodes a key
enzyme which is responsible for converting palmitic and stearic acids
into their monounsaturated forms. Additionally, the SCD1 protein was
shown to be involved in some aspects of energy homeostasis e.g.
lipogenesis, lipid oxidation, and thermo genesis. The SCD1 gene
is located on chromosome 26 in cattle. The blood samples of 394 dairy
cattle from four farms were used for extracting genomic DNA. A 400 bp
fragment of exon 5 of SCD1 gene was amplified by standard PCR, and
digested with NcoI enzyme for recognizing the SNP (C/T). The cytosine to
thymine substitution causes Alanine to change to Valine amino acids on
protein (A293V). The frequency of
AA, AV and VV were 0.6015, 0.3173 and 0.0812 respectively. The frequency
of alleles A and V were 0.76 and 0.24 respectively. The results of chi
square test showed that the population was not under Hardy Weinberg
equilibrium.
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Keywords:
Dairy cattle;
Polymorphism; stearoyl-coenzyme a desaturase 1 gene |
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To cite this article:
Mashhadi MH, S Sobhanirad, SE Nooraee and
RB Kashani,
2013.
Polymorphism of stearoyl-coenzyme a desaturase
1(SCD1) gene in Iranian holstein dairy cattle.
Res.
Opin.
Anim. Vet. Sci., 3(10), 363-365. |
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