The aim of this study was to determine the effects of gamma radiation and extrusion on chemical composition and electrophoresis of protein subunits patterns of corn and sorghum grains. Samples were extruded and irradiated at doses of 15, 30, and 45 kGy with gamma-rays. The results revealed that ash concentration was significantly high in corn and sorghum irradiated with 45 kGy and 30 kGy respectively. Ether extract and neutral detergent fibre was significantly high in extruded sorghum. Acid detergent lignin was significantly high in sorghum irradiated with 45 kGy. The results of this study showed that the gamma irradiation had the greatest impact in reducing corn and sorghum protein subunits, and the gamma radiation link the subunit of proteins which this characterize can be a good substitute for corn and sorghum in animal nutrition. The results of the present experiment concluded that extruding and gamma irradiation processing be reduced protein subunits MWs and could create positive effects on animal nutrition.