The RAPD-PCR method was used to screen the specific DNA markers for the black-dotted nose in Hanwoo. This study attempted to provide a tool to control the expression of the black-dotted nose in Hanwoo population in Korea. A total of 5 URP markers were used and analyzed the DNA variants among the 5 URP markers. A specific DNA marker was found in the URP 5 marker and named R5SNP. From the sequence analysis of R5SNP, 852 base sequences were detected. After compared with the registered DNA sequences in the data base, UMD 3.1 reference method used to check the homology of the base sequences between them and 99% of the sequence homology was detected. Also, a total of 11 SNPs variants found which were associated with the expression of the black-dotted nose. In our study, a specific segment of DNAs was found that was only in the black-dotted nose. This segment of DNAs was not associated with the normal yellow-coloured nose of Hanwoo. The R5SNP marker was found in this study could be used as an indicator for distinguishing Hanwoo and other foreign breeds and also for the early detection of the Black-dotted nose in Hanwoo.