This study was conducted to evaluate the genetic distances and specific DNA makers by the randomly amplified polymorphic DNA (RAPD)-PCR method for the black-dotted nose and the normal yellow nose in Hanwoo, the Korea native cattle. Genomic DNA was extracted from blood and nose tissues from Hanwoo after they were slaughtered. The extracted DNA was observed by nano-spectrometer. RAPD analysis was performed using 5 different primers. Statistical analysis was made for the estimation of the genetic distance among the cattle’s and the cluster tree was drawn by using MEGA 5.05 software. Genetic relations among them were determined by RAPD analysis. The polymorphic bands were observed 67% and the rest of 33% was monomorpic. The largest genetic distance (0.256) was found between the normal yellow nose and the black-dotted nose by UPGMAP method and the closest distance was observed between the black-dotted noses in Hanwoo as expected.