This study was conducted to determine the prevalence of Salmonella species in slaughtered animals and abattoir sewage from Zakho Abattoir, Kurdistan Region, Iraq. A total of 500 samples were collected including 200 from healthy sheep (100 rectal swabs and 100 gallbladder contents), 100 from healthy goats (50 rectal swabs and 50 gallbladder contents), 100 from cattle (50 rectal swabs and 50 gallbladder contents), and 100 sewage samples from the abattoir drains. From the total of 400 animals examined, 9 (2.25%) were positive for Salmonella spp., in which 5 (2.5%) were from sheep [ 2 (2%) from rectal swabs and 3 (3%) from gallbladder], 2 (2%) were from rectal swabs of goats, and 2 (2%) were from cattle [1 (2%) from rectal swabs and 1 (2%) from gallbladder]. Whereas, only 3 (3%) were positive for Salmonella spp., obtained from 100 sewage samples. Only three serotypes of Salmonella were detected in 12 (2.4%) samples from the total 500 samples. The isolated Salmonella serotypes were Salmonella hato 8 (66.66%), Salmonella anatum 3 (25%), and Salmonella enteritidis 1 (8.33%). The antimicrobial sensitivity test of all 12 isolates against 13 antibiotics was studied. Results revealed that all isolates were 100% sensitive to amoxicillin, amikacin, gentamicin, and ciprofloxacin, and 100% resistant to clindamycin, rifampin, vancomycin, cephalothin, lincomycin, and trimethoprim and sulfamethoxazole, chloramphinicol, doxycycline and tetracycline except S. hato.